目的:纤维蛋白封闭剂通常用于软骨碎片和植入物的关节内手术固定。然而,纤维蛋白封闭剂在软骨修复方面的机械性能尚不清楚。这项研究的目的是使用离体模型表征纤维蛋白密封剂的粘合和摩擦特性。
方法:用一次Tisseel或Vistaseal组装天然牛软骨-骨复合材料。在拉伸和搭接剪切下测试复合材料。此外,摩擦系数(COF)在单独的天然软骨环模型和软骨切碎的模型中进行测量。最后,评估了双重应用纤维蛋白密封剂的效果。
结果:拉伸模量没有显着差异,极限抗拉强度(UTS),剪切模量,或极限剪切强度(USS)之间的2纤维蛋白密封剂。两种纤维蛋白密封剂均显示UTS和USS<8和<30kPa,分别。当单独或与切碎的软骨一起测试时,密封剂之间的COF没有差异。与单次施用纤维蛋白密封剂相比,两次施用纤维蛋白密封剂不会改变机械性能。
结论:纤维蛋白密封剂粘合性能不受所研究的密封剂类型或在牛软骨-骨模型中的应用数量的影响。纤维蛋白密封剂的摩擦学特性不受密封剂类型或添加切碎的软骨的影响。Tisseel和Vistaseal的粘合性能低于软骨修复植入物体内固定所需的粘合性能。这些发现激发了用于软骨修复应用的改进的软骨特异性粘合剂的开发。
OBJECTIVE: Fibrin sealants are routinely used for intra-articular surgical fixation of cartilage fragments and implants. However, the mechanical properties of fibrin sealants in the context of cartilage repair are unknown. The purpose of this study was to characterize the adhesive and frictional properties of fibrin sealants using an ex vivo model.
METHODS: Native bovine cartilage-bone composites were assembled with a single application of Tisseel or
Vistaseal. Composites were tested in tension and lap shear. In addition, the coefficient of friction (COF) was measured in a native cartilage annulus model alone and with minced cartilage. Finally, the effect of a double application of fibrin sealant was evaluated.
RESULTS: There were no significant differences in tensile modulus, ultimate tensile strength (UTS), shear modulus, or ultimate shear strength (USS) between the 2 fibrin sealants. Both fibrin sealants demonstrated a UTS and USS of <8 and <30 kPa, respectively. There were no differences in COF between the sealants when tested alone or with minced cartilage. A double application of fibrin sealant did not alter the mechanical properties compared with a single application of fibrin sealant.
CONCLUSIONS: Fibrin sealant adhesive properties are not affected by the sealant type studied or the number of applications in a bovine cartilage-bone model. Fibrin sealant tribological properties are not affected by sealant type or the addition of minced cartilage. The adhesive properties of Tisseel and
Vistaseal were less than those desired for the in vivo fixation of cartilage repair implants. These findings motivate the development of an improved cartilage-specific adhesive for cartilage repair applications.