尽管正在进行控制努力,蚊子种群和由它们传播的疾病继续在世界范围内繁殖,引起重大健康问题。由于植物药具有广泛的杀虫特性,人们对使用植物药作为杀虫剂的替代品越来越感兴趣,生物降解性,和对生态条件的适应性。在这项研究中,我们研究了三种芳香植物-姜黄(姜黄)的溶剂提取物的杀幼虫活性和细胞毒性作用,美国罗勒(白罗勒),和Petroselinumcrispum(欧芹)-对抗白纹伊蚊。随后,我们通过气相色谱-质谱(GC-MS)分析了提取物的植物化学成分。结果表明,在处理后24小时,美洲和克里希姆的己烷提取物表现出最大的杀幼虫活性,中位致死浓度(LC50)值最低(<30µg/mL),发现前者对非洲猴肾(Vero)细胞的毒性大大降低。所述提取物的GC-MS分析表明存在不同种类的代谢物,包括苯丙素类化合物,非常长链的烷烃,脂肪酸及其衍生物,和萜烯,其中最丰富的成分是甲基丁香酚(55.28%),其中大部分,已经记录了他们的杀幼虫活动。这些发现为生物杀虫剂的潜在使用和开发提供了宝贵的见解,特别是来自美国。
Despite ongoing control efforts, the mosquito population and diseases vectored by them continue to thrive worldwide, causing major health concerns. There has been growing interest in the use of botanicals as alternatives to insecticides due to their widespread insecticidal properties, biodegradability, and adaptability to ecological conditions. In this study, we investigated the larvicidal activity and cytotoxicity effects of solvent extracts from three aromatic plants-Curcuma longa (turmeric), Ocimum americanum (hoary basil), and Petroselinum crispum (parsley)-against Aedes albopictus. Subsequently, we examined the phytochemical composition of the extracts through gas chromatography-mass spectrometry (GC-MS) analysis. Results revealed that the hexane extracts of O. americanum and P. crispum exhibited the greatest larvicidal activity with the lowest median lethal concentration (LC50) values (<30 µg/mL) at 24 h post-treatment, with the former found to be significantly less toxic towards African monkey kidney (Vero) cells. The GC-MS analysis of the said extract indicated the presence of different classes of metabolites, including phenylpropanoids, very long-chain alkanes, fatty acids and their derivatives, and terpenes, with the most abundant component being methyl eugenol (55.28%), most of which, have been documented for their larvicidal activities. These findings provide valuable insights into the potential use and development of bioinsecticides, particularly from O. americanum.