在这项研究中,我们使用来自绿色大型藻类Ulvafenestrata的多糖ulvan制备用于慢性糖尿病伤口愈合的水凝胶。使用ulvan二醛制备了基于天然多糖的水凝胶基质（UC-DPA-Ag水凝胶），壳聚糖,多巴胺（DPA）和银纳米颗粒（AgNPs）。将人脐带间充质干细胞冻干粉（hUC-MSCs）加载到水凝胶中以开发新型慢性糖尿病伤口愈合材料（UC-DPA-Ag@hUC-MSCs）。所得的水凝胶具有足够的机械性能，膨胀能力，粘附性,抗氧化剂，抗菌能力，促进细胞增殖和迁移。在II型糖尿病小鼠伤口模型中的体内伤口愈合表明，负载UC-DPA-Ag水凝胶的hUC-MSCs可以有效地促进伤口愈合。这种先进的水凝胶为糖尿病慢性伤口管理提供了一种简单有效的方法。此外，它为利用Ulva作为有价值的生物材料在全球和大规模生产有价值的添加生物材料提供了新的途径。
In this study, we used the polysaccharide ulvan from the green macroalgae Ulva fenestrata to prepare the hydrogel for chronic diabetic wound healing. A natural polysaccharide-based hydrogel matrix (UC-DPA-Ag hydrogel) was prepared using ulvan dialdehyde, chitosan, dopamine (DPA) and silver nanoparticles (Ag NPs). Human umbilical cord mesenchymal stem cell lyophilized powder (hUC-MSCs) was loaded into the hydrogel to develop a novel chronic diabetic wound healing material (UC-DPA-Ag@hUC-MSCs). The resulting hydrogel features adequate mechanical properties, swelling capability, adhesiveness, antioxidant, antibacterial ability, and promoting cell proliferation and migration. In vivo wound healing in type II diabetic mellitus mouse wound model showed that hUC-MSCs loaded UC-DPA-Ag hydrogel could accelerate wound healing effectively. This advanced hydrogel provides a facile and effective way for diabetic chronic wound management. Furthermore, it offers a new route for the utilizing Ulva as a valuable biomaterial for the global and large-scale production of valued added biomaterials.
