黄杆菌是藻类繁殖过程中的优势和活性细菌,在多糖降解中起重要作用。然而,对感染黄杆菌的噬菌体知之甚少,尤其是在绿潮期间。在这项研究中,一种新的病毒,vB_TgeS_JQ,从青岛金沙滩的地表水中分离出感染黄杆菌,中国。透射电镜显示vB_TgeS_JQ具有乙型病毒形态。实验表明,它在-20°C至45°C和pH5至pH8之间是稳定的,潜伏期和爆发期均持续20分钟。基因组分析表明,噬菌体vB_TgeS_JQ含有40,712-bp的dsDNA基因组,GC含量为30.70%,编码74个开放阅读框。确定了四个推定的辅助代谢基因,编码电子转移-黄素蛋白脱氢酶,钙调磷酸酶样磷酸酯酶,磷酸核糖-ATP焦磷酸水解酶,和TOPRIM核苷酸水解酶.Ulva增殖期间噬菌体vB_TgeS_JQ的丰度较高(U.增殖)与其他海洋环境相比。系统发育和比较基因组分析显示,vB_TgeS_JQ与数据库中的所有其他噬菌体分离株有显著差异,因此被归类为未被发现的病毒家族。名为Zblingviridae。总之,这项研究扩展了关于基因组的知识,黄细菌噬菌体的系统发育多样性和分布,尤其是他们在U.prolifera开花期间的角色。
目的:噬菌体vB_TgeS_JQ是绿潮中分离出的第一个黄细菌噬菌体,代表Caudoviricetes中的一个新家族,名为Zblingviridae。在Ulva增殖开花期间,噬菌体vB_TgeS_JQ的丰度较高。这项研究提供了对基因组的见解,系统发育多样性,和噬菌体的分布,尤其是他们在U.prolifera开花期间的角色。
Flavobacteriia are the dominant and active bacteria during algal blooms and play an important role in polysaccharide degradation. However, little is known about phages infecting Flavobacteriia, especially during green tide. In this study, a novel virus, vB_TgeS_JQ, infecting Flavobacteriia was isolated from the surface water of the Golden Beach of Qingdao, China. Transmission electron microscopy demonstrated that vB_TgeS_JQ had the morphology of siphovirus. The experiments showed that it was stable from -20°C to 45°C and pH 5 to pH 8, with latent and burst periods both lasting for 20 min. Genomic analysis showed that the phage vB_TgeS_JQ contained a 40,712-bp dsDNA genome with a GC content of 30.70%, encoding 74 open-reading frames. Four putative auxiliary metabolic genes were identified, encoding electron transfer-flavoprotein dehydrogenase, calcineurin-like phosphoesterase, phosphoribosyl-ATP pyrophosphohydrolase, and TOPRIM nucleotidyl hydrolase. The abundance of phage vB_TgeS_JQ was higher during Ulva prolifera (U. prolifera) blooms compared with other marine environments. The phylogenetic and comparative genomic analyses revealed that vB_TgeS_JQ exhibited significant differences from all other phage isolates in the databases and therefore was classified as an undiscovered viral family, named Zblingviridae. In summary, this study expands the knowledge about the genomic, phylogenetic diversity and distribution of flavobacterial phages (flavophages), especially their roles during U. prolifera blooms.
OBJECTIVE: The phage vB_TgeS_JQ was the first flavobacterial phage isolated during green tide, representing a new family in Caudoviricetes and named Zblingviridae. The abundance of phage vB_TgeS_JQ was higher during the Ulva prolifera blooms. This study provides insights into the genomic, phylogenetic diversity, and distribution of flavophages, especially their roles during U. prolifera blooms.