背景:小野草。是一种短命的常年冷季豆科植物,用于饲料生产。它是小亚细亚常见的本地物种,尤其是在土耳其,高加索地区,还有里海边缘.它可以在亚洲广泛的气候和土壤类型中生长良好,欧洲,和北美。它是一种不膨胀的作物,使其适用于干草和牧场。
结果:目的是评估根据TekirdagNamikKemal大学实验场评估的种质资源中的高农艺性能选择的83种三苯膦基因型的多样性,土耳其。研究中使用了十种核简单序列重复(nSSR)引物(OVK036,OVK046,OVK094,OVK101,OVK125,OVK161,OVK174,OVM033,OVM061和OVM125)。发现所有nSSR基因座均为多态性,共检测到92个等位基因。每个基因座的平均观察到的等位基因数计算为9.2。在遗传多样性参数中,香农指数(I=0.375),无偏遗传多样性值(Uh=0.243),并计算平均多态信息含量(PIC=0.240)。遗传距离值在0.43和0.95之间变化。基于UPGMA聚类方法利用遗传距离值构建的树状图,观察到所研究的sainfoin基因型分为两个主要簇,而结构分析结果对三个集群有很高的支持。
结论:这项研究获得的结果提供了有关所研究的sainfoin基因型的遗传结构及其遗传关系的重要信息。因此,获得的遗传数据将有助于设计更有效的多交苗圃,允许在分离条件下对最佳表现和遗传多样性的基因型进行开放授粉,这将增加赛恩福因育种计划的遗传增益。
BACKGROUND: Onobrychis viciifolia Scop. is a short-lived perennial cool-season legume used for forage production. It is a common native species in Asia Minor, especially in Turkey, the districts of the Caucasus, and the Caspian fringes. It can grow well in a broad range of climatic and soil types found in Asia, Europe, and North America. It is a non-bloating crop, making it suitable for use in both hay and pasture.
RESULTS: The aim was to assess the diversity of the 83 sainfoin genotypes selected based on their high agronomic performance from a germplasm collection evaluated in the experimental field of Tekirdag Namik Kemal University, Turkey. Ten nuclear simple sequence repeat (nSSR) primers (OVK036, OVK046, OVK094, OVK101, OVK125, OVK161, OVK174, OVM033, OVM061, and OVM125) were used in the study. All nSSR loci were found to be polymorphic and totally 92 alleles were detected. The mean observed number of alleles per locus was calculated as 9.2. Among the genetic diversity parameters, Shannon Index (I = 0.375), unbiased genetic diversity value (uh = 0.243), and mean polymorphic information content (PIC = 0.240) were calculated. The genetic distance value varied between 0.43 and 0.95. Based on the dendrogram built by the
UPGMA clustering method using genetic distance values, it was observed that the studied sainfoin genotypes were divided into two main clusters, whereas the STRUCTURE analysis results had high support for three clusters.
CONCLUSIONS: The results obtained from this study provide important information on the genetic structures of the studied sainfoin genotypes and their genetic relationship. Therefore acquired genetic data will be useful in designing more efficient polycross nurseries, allowing open pollination of best performing and genetically diverse genotypes in the isolated conditions, which will increase genetic gain in sainfoin breeding programs.