UNASSIGNED:对疾病与基因之间关系的研究以及遗传分析技术的进步使医疗保健中的基因检测成为可能。基因检测有多种方法,包括基于PCR的方法和下一代测序;然而,临床实验室的筛查测试变得越来越多样化;因此,需要新颖的测量系统和设备来满足每种情况的需求。在这项研究中,我们的目标是开发一种新型的基于微阵列的遗传分析系统,该系统使用Peltier元件来克服传统微阵列的问题,如测量时间长,成本高。
UNASSIGNED:我们构建了一个微阵列系统,以检测有资格接受盐酸伊立替康治疗的患者的UDP-葡糖醛酸基转移酶基因多态性UGT1A1*6和UGT1A1*28,用于临床实验室。为了评估系统的性能,确定杂交温度和反应时间,并将结果与使用常规杂交炉获得的结果进行了比较。
UNASSIGNED:杂交温度在常规系统所需时间的1/27内达到其目标。我们评估了111个人类临床样本,发现我们的结果与使用现有方法获得的结果一致。与现有方法相比,新开发设备的总时间减少了85分钟,因为自动化的DNA微阵列消除了现有方法花费在手工操作上的时间。
UNASSIGNED:我们系统中使用的表面处理技术可实现高密度和牢固的DNA固定,允许构建适合临床应用的测量系统。
UNASSIGNED: Research on the relationship between diseases and genes and the advancement of genetic analysis technologies have made genetic testing in medical care possible. There are various methods for genetic testing, including PCR-based methods and next-generation sequencing; however, screening tests in clinical laboratories are becoming more diverse; therefore, novel measurement systems and equipment are required to meet the needs of each situation. In this study, we aimed to develop a novel microarray-based genetic analysis system that uses a Peltier element to overcome the issues of conventional microarrays, such as the long measurement time and high cost.
UNASSIGNED: We constructed a microarray system to detect the UDP-glucuronosyltransferase gene polymorphisms UGT1A1*6 and UGT1A1*28 in patients eligible for irinotecan hydrochloride treatment for use in clinical laboratories. To evaluate the performance of the system, the hybridization temperature and reaction time were determined, and the results were compared with those obtained using a conventional hybridization oven.
UNASSIGNED: The hybridization temperature reached its target in 1/27th of the time required by the conventional system. We assessed 111 human clinical samples and found that our results agreed with those obtained using existing methods. The total time for the newly developed device was reduced by 85 min compared to that for existing methods, as the automated DNA microarray eliminates the time that existing methods spend on manual operation.
UNASSIGNED: The surface treatment technology used in our system enables high-density and strong DNA fixation, allowing the construction of a measurement system suitable for clinical applications.