背景:子宫内膜损伤被认为是女性不孕的主要原因。传统疗法如雌激素替代疗法由于治疗反应的个体差异而不能令人满意,因此,有必要使用替代策略,如干细胞治疗。干细胞移植,如脐带间充质干细胞(UCMSCs),已被证明可以改善子宫内膜愈合。然而,由于宫内环境的影响,UCMSCs的治疗效果有限,其功效不稳定。HOXA10,由HOXA10基因编码,在子宫内膜形态维持中起着重要作用,扩散,分化,和胚胎植入。此外,UCMSC不显示HOXA10表达。
目的:我们的研究旨在评估HOXA10转染的UCMSCs对子宫内膜损伤修复的治疗作用。
方法:首先,我们建立了T10-UCMSCs(用HOXA10转染的UCMSCs)用于移植。建立子宫内膜损伤模型,我们将95%的乙醇注入子宫腔,并将T10-UCMSCs从子宫角向子宫腔移植。十四天后,收集子宫用于子宫内膜生长和容受性的组织学和生化分析。
结果:我们的结果表明T10-UCMSCs组子宫内膜容受性优于UCMSCs组,提示HOXA10可增强UCMSCs在子宫内膜损伤修复中的修复能力。更重要的是,育性试验显示T10-UCMSCs组植入了更多的胚胎.
结论:我们的结果表明,表达HOXA10的UCMSCs可以改善子宫内膜损伤修复的治疗效果。
BACKGROUND: Endometrial injury is considered the major cause of female infertility. Traditional therapies such as estrogen substitution therapy are not satisfactory due to individual variation in response to treatment, thereby warranting the use of alternative strategies such as stem cell therapy. Transplantation of stem cells, such as umbilical cord mesenchymal stem cells (
UCMSCs), has been shown to improve endometrial healing. However, due to the effect of the intrauterine environment, the therapeutic effect of
UCMSCs is limited, and its efficacy is unstable. HOXA10, encoded by the HOXA10 gene, plays an important role in endometrium morphology maintenance, proliferation, differentiation, and embryo implantation. Moreover,
UCMSCs do not show HOXA10 expression.
OBJECTIVE: Our study aimed to evaluate the therapeutic effects of HOXA10-transfected UCMSCs on endometrial injury repair in vivo.
METHODS: First, we established T10-
UCMSCs (
UCMSCs transfected with HOXA10) for transplantation. To establish the endometrial injury model, we injected 95% ethanol into the uterine cavity and transplanted T10-UCMSCs into the uterine cavity from the cornua uteri. Fourteen days later, uteri were collected for histological and biochemical analysis of endometrial growth and receptivity.
RESULTS: Our results showed the endometrial receptivity was better in T10-UCMSCs group than in
UCMSCs group, suggesting that HOXA10 could enhance the repairing ability of
UCMSCs in the endometrium injury repair. More importantly, the fertility test showed that more embryos were implanted in the T10- UCMSCs group.
CONCLUSIONS: Our results suggest that UCMSCs with HOXA10 expression could improve the therapeutic effects on endometrial injury repairing.