背景:内膜增生是动脉对损伤的正常适应性特征,其中包括侵入性血管干预。它的发展限制了旁路移植的长期成功。已经在实验模型中成功地采用了各种药理学试剂来降低内膜增生的程度。在我们的研究中,我们研究了地塞米松对大鼠腹主动脉部分横切和一期修复后内膜增生的疗效。
方法:在本研究中,随机选择20只Wistar白化病大鼠,分为四组,比较低剂量和高剂量地塞米松与对照组相比对内膜和中膜厚度的影响。A组(n=5)为对照组,仅进行皮肤切开和剖腹手术。对于B组(n=5),进行了正中剖腹手术,腹主动脉部分横断,并用8.0prolene缝线修复。C组(n=5)和D组(n=5)给予0.1mg/kg和0.2mg/kg地塞米松的剂量,分别。两周后,所有的老鼠都被安乐死,切除修复后的腹主动脉并进行组织病理学检查。使用“OlympusAnalySIS5”程序测量内膜和介质厚度(OlympusCorporation,日本)拍摄数码照片后。
结果:根据测量结果,我们证明了在横切和修复腹主动脉后,低剂量地塞米松组和非地塞米松组的内膜/中膜比值无显著差异.高剂量地塞米松组的内膜/中膜比率明显低于非地塞米松和低剂量地塞米松组。
结论:血管干预后,地塞米松治疗可通过提供血管重塑减少内膜增生并增加通畅性.
BACKGROUND: Intimal hyperplasia is a normal adaptive feature of arteries in response to injuries, which include invasive vascular interventions. Its development limits the long-term success of bypass grafts. Various pharmacological agents have been successfully employed in experimental models to reduce the degree of intimal hyperplasia. In our study, we investigated the efficacy of dexamethasone in reducing intimal hyperplasia in rat abdominal aortas after partial transection and primary repair.
METHODS: In this study, 20 Wistar Albino rats were randomly selected and divided into four groups to compare the effects of low- and high-dose dexamethasone on intima and media thickness compared to the control. Group A (n=5) was the control group, where only skin incision and laparotomy were performed. For Group B (n=5), a median laparotomy was performed, the abdominal aorta was partially transected, and repaired with an 8.0 prolene suture. Doses of 0.1 mg/kg and 0.2 mg/kg dexamethasone were administered in Group C (n=5) and Group D (n=5), respectively. After two weeks, all rats were euthanized, and the repaired abdominal aortas were excised and examined histopathologically. Intima and media thicknesses were measured using the \'Olympus AnalySIS 5\' program (Olympus Corporation, Japan) after digital photos were taken.
RESULTS: Based on the measurements, we demonstrated that after transection and repair of the abdominal aorta, the intima/media ratio was not significantly different between the low-dose dexamethasone and non-dexamethasone groups. The intima/media ratio was significantly lower in the high-dose dexamethasone group than in the non-dexamethasone and low-dose dexamethasone groups.
CONCLUSIONS: After vascular interventions, dexamethasone treatment may reduce intimal hyperplasia and increase patency by providing vascular remodeling.