未经批准:小儿神经胶质瘤包括一组不同的脑肿瘤实体,对患者的生存和生活质量有实质性的长期影响。然而,由于缺乏真实的模型,目前对这些肿瘤的研究有限。此外,小儿脑肿瘤生物学的许多方面,如肿瘤细胞侵袭,用目前可用的工具很难研究。为了解决这些问题,我们开发了一种基于合成细胞外基质(sECM)的培养系统,以生长和研究原发性小儿脑肿瘤细胞。
UNASSIGNED:我们开发了一种类似大脑的sECM材料,作为原代培养的支撑支架,患者来源的小儿神经胶质瘤细胞和建立的患者来源的细胞系。原代幼年脑干衍生的鼠星形胶质细胞用作饲养层,以支持原代人肿瘤细胞的生长。
UNASSIGNED:我们发现我们的培养系统促进了各种原发性小儿脑肿瘤的增殖,包括低度胶质瘤,并启用了研究性治疗剂的离体测试。此外,我们发现,调整这种sECM材料使我们能够评估高级儿科神经胶质瘤细胞侵袭性,并评估针对侵袭行为的治疗干预措施.
UNASSIGNED:我们的sECM培养平台为儿科脑肿瘤研究人员提供了一种多用途工具,可以进行广泛的生物学检测和培养不同类型的肿瘤。
UNASSIGNED: Pediatric gliomas comprise a diverse set of brain tumor entities that have substantial long-term ramifications for patient survival and quality of life. However, the study of these tumors is currently limited due to a lack of authentic models. Additionally, many aspects of pediatric brain tumor biology, such as tumor cell invasiveness, have been difficult to study with currently available tools. To address these issues, we developed a synthetic extracellular matrix (sECM)-based culture system to grow and study primary pediatric brain tumor cells.
UNASSIGNED: We developed a brain-like sECM material as a supportive scaffold for the culture of primary, patient-derived pediatric glioma cells and established patient-derived cell lines. Primary juvenile brainstem-derived murine astrocytes were used as a feeder layer to support the growth of primary human tumor cells.
UNASSIGNED: We found that our culture system facilitated the proliferation of various primary pediatric brain tumors, including low-grade gliomas, and enabled ex vivo testing of investigational therapeutics. Additionally, we found that tuning this sECM material allowed us to assess high-grade pediatric glioma cell invasion and evaluate therapeutic interventions targeting invasive behavior.
UNASSIGNED: Our sECM culture platform provides a multipurpose tool for pediatric brain tumor researchers that enables both a wide breadth of biological assays and the cultivation of diverse tumor types.