低剂量辐射暴露的影响仍然是辐射生物学中一个有争议的话题。这项研究比较了γH2AX的早期(0.5、4、24、48和72小时)和晚期(5、10和15个细胞传代)辐照后变化,53BP1,pATM,和p-p53(Ser-15)病灶,扩散,自噬,和暴露于100和2000mGyX射线辐射的原代成纤维细胞的衰老。结果表明,暴露于100mGy显著增加γH2AX,53BP1,而pATM病灶仅在辐照后0.5和4小时。p-p53(Ser-15)病灶无变化,扩散,自噬,或在低剂量照射后衰老多达15个传代。
The effects of low-dose radiation exposure remain a controversial topic in radiation biology. This study compares early (0.5, 4, 24, 48, and 72 h) and late (5, 10, and 15 cell passages) post-irradiation changes in γH2AX, 53BP1, pATM, and p-p53 (Ser-15) foci, proliferation, autophagy, and senescence in primary fibroblasts exposed to 100 and 2000 mGy X-ray radiation. The results show that exposure to 100 mGy significantly increased γH2AX, 53BP1, and pATM foci only at 0.5 and 4 h post irradiation. There were no changes in p-p53 (Ser-15) foci, proliferation, autophagy, or senescence up to 15 passages post irradiation at the low dose.