背景:胃癌(GC)是全球最普遍的癌症类型之一,保持癌症相关死亡的第二大原因的位置。胃病变代表胃粘膜的病理性改变,有进展为胃癌的倾向.有限的研究探索了干细胞在治疗胃病变中的潜力。
方法:本研究旨在探讨标记的骨髓间充质干细胞(BMMSCs)静脉移植抑制胃癌前病变进展的潜力。
结果:在胃病变疾病模型组中,大鼠组织表现出明显的粘膜萎缩,肠上皮化生,发育不良,和炎性细胞浸润。输注BMMSCs后,发现胃部病变明显减少,萎缩性胃炎是唯一残留的病变,通过形态学和组织学检查证实。定植在胃部病变处的BMMSCs可以分化为上皮细胞和基质细胞,由泛角蛋白或波形蛋白的表达确定。BMMSC移植后血管内皮生长因子的表达显着升高。BMMSCs还可以上调体液免疫应答细胞因子的产生,包括白细胞介素(IL)-4和IL-10,并下调IL-17和干扰素-γ的产生,这可能与细胞免疫反应和病变的炎症严重程度高度相关。
结论:BMMSC移植可显着减轻炎症并逆转胃部病变的进展。
BACKGROUND: Gastric cancer (GC) stands as one of the most prevalent cancer types worldwide, holding the position of the second leading cause of cancer-related deaths. Gastric lesions represent pathological alterations to the gastric mucosa, with an elevated propensity to advance to gastric cancer. Limited research has explored the potential of stem cells in the treatment of gastric lesions.
METHODS: This study aimed to explore the potential of intravenous transplantation of labeled bone marrow-derived mesenchymal stem cells (BMMSCs) to inhibit the progression of precancerous gastric lesions.
RESULTS: In the gastric lesion disease model group, the rat tissue exhibited noteworthy mucosal atrophy, intestinal metaplasia, dysplasia, and inflammatory cell infiltration. Following the infusion of BMMSCs, a notable decrease in gastric lesions was found, with atrophic gastritis being the sole remaining lesion, which was confirmed by morphological and histological examinations. BMMSCs that were colonized at gastric lesions could differentiate into epithelial and stromal cells, as determined by the expression of pan-keratin or vimentin. The expression of vascular endothelial growth factor was significantly elevated following BMMSC transplantation. BMMSCs could also upregulate the production of humoral immune response cytokines, including interleukin (IL)-4 and IL-10, and downregulate the production of IL-17 and interferon-gamma, which could be highly associated with the cellular immune response and inflammation severity of the lesions.
CONCLUSIONS: BMMSC transplantation significantly reduced inflammation and reversed gastric lesion progression.