对Aphanamixispolystachya茎皮甲醇提取物的植物化学研究导致分离出四个先前未描述的化合物(1-4)和十个已知的化合物(5-14)。它们的化学结构被阐明为11-甲氧基夸拉螺C(1),6α,9S,10,13-四羟基megastigmane-3-酮(2),11-羟基阿帕尼辛B(3),(2Z,6E,13E)-2,6,13-三烯-11,15-二羟基植酸(4),cinnacassideD(5),cinnacassideE(6),vilsonolF(7),(3S,5R,6S,7E,9R)-3,5,6,9-四羟基-7-烯-megastigmane(8),(3S,5R,6R,7E,9R)-3,6,9,10-四羟基-7-烯-megastigmane(9),柑橘苷A(10),苏-1-(3,4,5-三甲氧基苯基)-1,2,3-丙三醇(11),3,4,5-三甲氧基苯基-1-O-β-D-吡喃葡萄糖苷(12),对香豆酸(13),阿魏酸(14)通过HR-ESI-MS,ECD,1D-,和2D-NMR光谱。化合物1、3、4和9在LPS激活的RAW264.7细胞中显示出NO产生抑制活性,IC50值为42.0、67.9、20.5和78.6μM,分别,而其余化合物无活性,IC50值超过100μM。
Phytochemical study on the methanol extract of the stem barks of Aphanamixis polystachya led to the isolation of four previously undescribed (1-4) and ten known compounds (5-14). Their chemical structures were elucidated to be 11-methoxysawaranospiroride C (1), 6α,9S,10,13-tetrahydroxymegastigmane-3-one (2), 11-hydroxyaphanamixin B (3), (2Z,6E,13E)-2,6,13-triene-11,15-dihydroxyphytanic acid (4), cinnacasside D (5), cinnacasside E (6), vilsonol F (7), (3S,5R,6S,7E,9R)-3,5,6,9-tetrahydroxy-7-en-megastigmane (8), (3S,5R,6R,7E,9R)-3,6,9,10-tetrahydroxy-7-en-megastigmane (9), citroside A (10), threo-1-(3,4,5-trimethoxyphenyl)-1,2,3-propanetriol (11), 3,4,5-trimethoxyphenyl-1-O-β-D-glucopyranoside (12), p-coumaric acid (13), ferulic acid (14) by HR-ESI-MS, ECD, 1D-, and 2D-NMR spectra. Compounds 1, 3, 4, and 9 showed NO production inhibitory activity in LPS activated RAW 264.7 cells with IC50 values of 42.0, 67.9, 20.5, and 78.6 μM, respectively, while the remaining compounds were inactive with IC50 values over 100 μM.