我们对来自25种木本植物物种的253种蛋白质序列(Pfam00257)进行了分析,包括树木,灌木,和葡萄藤。我们的目标是深入了解他们的建筑类型,生化特性,以及可能参与减轻非生物胁迫,比如干旱,冷,或盐度。研究的蛋白质序列(253)包括221个被子植物(85棵树/灌木和36棵藤本植物)和32个裸子植物。我们的序列分析揭示了七种建筑类型的存在:Kn,KnS,SKn,YnKn,YnSKn,FSKn,还有FnKn.FSKn型在裸子植物和被子植物的树和灌木脱水素中占主导地位,而YnSKn型在葡萄脱水素中更为普遍。裸子植物中不存在YnSKn和YnKn类型。裸子植物脱水蛋白表现出向更负的GRAVY评分和折叠指数的转变。此外,他们表现出更高的Lys含量和更低的His含量。通过分析被子植物物种的启动子序列,包括树木,灌木,和葡萄藤,我们发现这些脱水蛋白是由ABA依赖性和光响应途径诱导的。压力和激素相关的顺式元素的存在表明对脱水的保护作用,冷,或盐度。这些发现可以作为木本脱水素未来研究的基础,特别是在生物技术应用的背景下。
We conducted analyses on 253 protein sequences (Pfam00257) derived from 25 woody plant species, including trees, shrubs, and vines. Our goal was to gain insights into their architectural types, biochemical characteristics, and potential involvement in mitigating abiotic stresses, such as drought, cold, or salinity. The investigated protein sequences (253) comprised 221 angiosperms (85 trees/shrubs and 36 vines) and 32 gymnosperms. Our sequence analyses revealed the presence of seven architectural types: Kn, KnS, SKn, YnKn, YnSKn, FSKn, and FnKn. The FSKn type predominated in tree and shrub dehydrins of both gymnosperms and angiosperms, while the YnSKn type was more prevalent in vine dehydrins. The YnSKn and YnKn types were absent in gymnosperms. Gymnosperm dehydrins exhibited a shift towards more negative GRAVY scores and Fold Indexes. Additionally, they demonstrated a higher Lys content and lower His content. By analyzing promoter sequences in the angiosperm species, including trees, shrubs, and vines, we found that these dehydrins are induced by the ABA-dependent and light-responsive pathways. The presence of stress- and hormone-related cis-elements suggests a protective effect against dehydration, cold, or salinity. These findings could serve as a foundation for future studies on woody dehydrins, especially in the context of biotechnological applications.