诱导多能干(iPS)细胞由体细胞产生并且可以分化成各种细胞类型。因此,这些细胞有望成为模拟疾病和移植治疗的有力工具。已经报道了依赖于白血病抑制因子的家猫iPS细胞的产生;然而,此策略可能无法优化。考虑到家猫是研究自发性疾病的优秀模型,iPS细胞生成至关重要。在这项研究中,我们旨在从逆转录病毒转染小鼠Oct3/4,Klf4,Sox2和c-Myc的猫胚胎成纤维细胞中获得iPS细胞。转染后,将胚胎成纤维细胞重新接种到灭活的SNL76/7上,并在补充有碱性成纤维细胞生长因子的培养基中培养。扁平,紧凑型,观察到类似人iPS集落的原代集落。此外,与胎牛血清(FBS)培养基相比,在敲除血清替代培养基中更频繁地观察到原始菌落。然而,FBS培养基中iPS样细胞的维持和增殖增强。这些iPS样细胞表达胚胎干细胞标志物,核型正常,增殖超过45个通道,并在体外分化为所有三个胚层。值得注意的是,外源Oct3/4、Klf4和Sox2的表达在这些细胞中被沉默。然而,iPS样细胞未能形成畸胎瘤。总之,这是第一个建立和表征猫iPS样细胞的研究,它可以根据碱性成纤维细胞生长因子分化为不同的细胞类型。
Induced pluripotent stem (iPS) cells are generated from somatic cells and can differentiate into various cell types. Therefore, these cells are expected to be a powerful tool for modeling diseases and transplantation therapy. Generation of domestic cat iPS cells depending on leukemia inhibitory factor has been reported; however, this strategy may not be optimized. Considering that domestic cats are excellent models for studying spontaneous diseases, iPS cell generation is crucial. In this study, we aimed to derive iPS cells from cat embryonic fibroblasts retrovirally transfected with mouse Oct3/4, Klf4, Sox2, and c-Myc. After transfection, embryonic fibroblasts were reseeded onto inactivated SNL 76/7 and cultured in a medium supplemented with basic fibroblast growth factor. Flat, compact, primary colonies resembling human iPS colonies were observed. Additionally, primary colonies were more frequently observed in the KnockOut Serum Replacement medium than in the fetal bovine serum (FBS) medium. However, enhanced maintenance and proliferation of iPS-like cells occurred in the FBS medium. These iPS-like cells expressed embryonic stem cell markers, had normal karyotypes, proliferated beyond 45 passages, and differentiated into all three germ layers in vitro. Notably, expression of exogenous Oct3/4, Klf4, and Sox2 was silenced in these cells. However, the iPS-like cells failed to form teratomas. In conclusion, this is the first study to establish and characterize cat iPS-like cells, which can differentiate into different cell types depending on the basic fibroblast growth factor.