将JulesGuyot梨浸入acibilolar-S-甲基(ASM)和0.01molL-1乙基乙二醇四乙酸(EGTA)中,以研究Ca2受体蛋白和苯丙素途径的变化。结果表明,ASM处理增加了苯丙氨酸解氨酶(PAL)的活性,肉桂酸-4-羟化酶(C4H),4-香豆酸辅酶A连接酶(4CL),多酚氧化酶(PPO),梨外皮中的肉桂醇脱氢酶(CAD),而EGTA预处理抑制了这些酶的活性。ASM治疗也增强了PcPAL的转录,PcC4H,Pc4CL,PcC3H,PcCOMT,PcCCoAOMT,PCCCR,PcPOD,梨中的PcCDPK1、PcCDPK2、PcCDPK5、PcCDPK11、PcCDPK13、PcCBL1、PcCBL9、PcCIPK14和PcCML27。EGTA+ASM处理抑制了PcPAL的转录,PcC4H,Pc4CL,PcC3H,PCCCR,PcF5H,PcCAD,果实中PcCDPK11、PcCDPK26、PcCDPK32、PcCBL1、PcCIPK14、PcCIPK23和PcCaM。所有这些结果表明ASM诱导了Ca2受体蛋白的基因表达,苯丙素途径中关键酶活性和基因表达;Ca2+介导梨体内苯丙素代谢。
\'Docteur Jules Guyot\' pears were immersed in acibenzolar-S-methyl (ASM) and 0.01 mol L-1 ethyl glycol tetra acetic acid (EGTA) to investigate the changes of Ca2+ receptor proteins and phenylpropanoid pathway. Results showed that ASM treatment increased the activities of phenylalanine ammonia-lyase (PAL), cinnamate-4-hydroxylase (C4H), 4-coumarate coenzyme A ligase (4CL), polyphenol oxidase (PPO), and cinnamyl alcohol dehydrogenase (CAD) in the exocarp of pears, whereas EGTA pre-treatment inhibited the activities of these enzymes. ASM treatment also enhanced the transcription of PcPAL, PcC4H, Pc4CL, PcC3H, PcCOMT, PcCCoAOMT, PcCCR, PcPOD, PcCDPK1, PcCDPK2, PcCDPK5, PcCDPK11, PcCDPK13, PcCBL1, PcCBL9, PcCIPK14, and PcCML27 in pears. EGTA + ASM treatments inhibited the transcription of PcPAL, PcC4H, Pc4CL, PcC3H, PcCCR, PcF5H, PcCAD, PcCDPK11, PcCDPK26, PcCDPK32, PcCBL1, PcCIPK14, PcCIPK23, and PcCaM in the fruit. All these results indicated that ASM induced the gene expressions of Ca2+ receptor proteins, the key enzyme activities and gene expressions in phenylpropanoid pathway; Ca2+ mediated phenylpropane metabolism in pears after ASM treatment.