Bopyeo-tang(BPT)由六种草药组成(桑树,地黄(Gaertn。)DC。,人参C.A.Mey.,AstertataricusL.f.,黄芪proposquusSchischkin,和五味子(Turcz。)Baill。),并已用于治疗肺部疾病。这项研究的重点是建立一种可以同时量化9种目标化合物的分析方法(即,羟甲基糠醛,MulberrosideA,绿原酸,calycosin-7-O-葡萄糖苷,3,5-二咖啡酰基奎尼酸,槲皮素,山奈酚,Schizandrin,和gomisinA)来自BPT样品,使用带有光电二极管阵列检测器的高效液相色谱(HPLC-PDA)和带有串联质谱的超高效液相色谱(UPLC-MS/MS)。两种分析中化合物的分离均在C18反相柱上进行,使用水-乙腈的梯度洗脱作为流动相。特别是,在UPLC-MS/MS分析中采用多反应监测模式进行快速、准确的检测。通过对这两种方法的分析,HPLC-PDA和UPLC-MS/MS,各化合物回归方程的测定系数≥0.9952,回收率为85.99-106.40%(相对标准偏差(RSD)<9.58%)。验证了9种化合物的精度测试(RSD<10.0%)。在优化条件下应用这些分析测定以定量分析BPT样品,得到0.01-4.70mg/g。因此,这两种检测方法可成功收集临床研究和BPT质量控制的基础数据。
Bopyeo-tang (BPT) is composed of six medicinal herbs (Morus alba L., Rehmannia glutinosa (Gaertn.) DC., Panax ginseng C.A.Mey., Aster tataricus L.f., Astragalus propinquus Schischkin, and Schisandra chinensis (Turcz.) Baill.) and has been used for the treatment of lung diseases. This study focused on establishing an analytical method that can simultaneously quantify nine target compounds (i.e., hydroxymethylfurfural, mulberroside A, chlorogenic acid, calycosin-7-O-glucoside, 3,5-dicaffeoylquinic acid, quercetin, kaempferol, schizandrin, and gomisin A) from a BPT sample using high-performance liquid chromatography with a photodiode array detector (HPLC-PDA) and ultra-performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS). The separation of compounds in both analyses was performed on a C18 reversed-phase column using the gradient elution of water-acetonitrile as the mobile phase. In particular, the multiple reaction monitoring mode was applied for quick and accurate detection in UPLC-MS/MS analysis. As a result of analyzing the two methods, HPLC-PDA and UPLC-MS/MS, the coefficient of determination of the regression equation for each compound was ≥0.9952, and recovery was 85.99-106.40% (relative standard deviation (RSD) < 9.58%). Precision testing of the nine compounds was verified (RSD < 10.0%). The application of these analytical assays under optimized conditions for quantitative analysis of the BPT sample gave 0.01-4.70 mg/g. Therefore, these two assays could be used successfully to gather basic data for clinical research and the quality control of BPT.