目标:最近的研究将影响人类和动物健康的新出现和重新出现的疾病列为优先事项,特别是描述这些疾病如何进入国家并确定其传播周期。鉴于候鸟在传播感染中起着重要作用,本研究分析了它们的迁移路径和标本,以调查正黄病毒,正负病毒,萨姆桑省鸟类中的甲病毒和猪病毒,蒂尔基耶.
结果:对于这些病毒,使用RT-PCR和qRT-PCR分析来自56只鸟的312个样品。在14只鸟中发现了托斯卡纳病毒(TOSV)(4只野鸭,五个part,四只鹌鹑和一只鸽子),代表25%的鸟类样本。在所有14只鸟中都报道了基因型B。在细胞培养物中接种阳性组织后,TOSV是从鸽子的器官中分离出来的,野鸭和part。
结论:这是首次从鸟类的细胞培养物中分离出TOSV,表明它们可能在Türkiye中传播TOSV中起作用。结果还表明,候鸟可能在国家之间携带TOSV。
OBJECTIVE: Recent research has prioritized emerging and re-emerging diseases that affect human and animal health, particularly to describe how these diseases enter countries and determine their transmission cycles. Given that migratory birds play a significant role in spreading infections, the present study analysed their migration paths and specimens to investigate Orthoflavivirus, Orthonairovirus, Alphavirus and Phlebovirus in birds in Samsun province, Türkiye.
RESULTS: For these viruses, 312 samples from 56 birds were analysed using RT-PCR and qRT-PCR. Toscana virus (TOSV) was identified in 14 birds (four mallards, five partridges, four quails and one pigeon), representing 25% of the birds sampled. Genotype B was reported in all 14 birds. After inoculating the positive tissues in cell cultures, TOSV was isolated from the organs of pigeons, mallards and partridges.
CONCLUSIONS: This is the first time TOSV has been isolated in cell culture from birds and indicates that they may play a role in spreading TOSV in Türkiye. The results also suggest that TOSV might be carried between countries by migratory birds.