■癌症患者死亡的重要原因之一是恶性转移,入侵,和肿瘤细胞的转移。转移也是各种肿瘤最基本的生理特征和发病机制。先前发表的研究表明,自分泌运动因子受体(AMFR)是肿瘤细胞迁移和侵袭的关键调节因子。同时,AMFR在食管肿瘤中高表达,胃肠道肿瘤,膀胱癌,它也参与了它的发病机理。然而,AMFR在胶质母细胞瘤中的作用尚未见报道.
■为了研究AMFR在胶质母细胞瘤细胞迁移和侵袭中的作用,使用siRNA沉默AMFR并使用cDNA过表达。免疫印迹分析和实时定量聚合酶链反应(PCR)用于评估AMFR的表达。我们进行了伤口愈合试验,细胞迁移试验,和瘤球形成试验检测胶质母细胞瘤的侵袭和转移能力。
■这项研究发现,临床样本中AMFR的表达水平与胶质瘤组织的恶性程度显着相关。AMFR沉默降低了LN229的细胞迁移和侵袭。过表达AMFR显著增加U251的细胞迁移和侵袭能力。
■本研究提示AMFR可作为胶质母细胞瘤临床治疗的治疗策略。
UNASSIGNED: One of the important causes of death in cancer patients is malignant metastasis, invasion, and metastasis of tumor cells. Metastasis is also the most basic physiological characteristics and pathogenesis of various tumors. Previously published studies have suggested that autocrine motor factor receptor (AMFR) is the key regulator of tumor cell migration and invasion. Meanwhile, AMFR is highly expressed in esophageal tumors, gastrointestinal tumors, and bladder cancer, and it is also involved in its pathogenesis. However, the role of AMFR in glioblastoma has not been reported.
UNASSIGNED: In order to study the role of AMFR in the cell migration and invasion of glioblastoma, AMFR was silenced using siRNA and overexpressed using cDNA. Immunoblotting analysis and real-time quantitative polymerase chain reaction (PCR) were employed to assess the expression of AMFR. We conducted wound healing assay, cell migration assay, and tumorsphere formation assay to detect the invasion and metastatic ability of glioblastoma.
UNASSIGNED: This study found that the level of AMFR expression was significantly correlated with the malignant degree of glioma tissue in clinic samples. AMFR silencing decreased cell migration and invasion of LN229. Overexpression of AMFR significantly increased cell migration and invasion of U251.
UNASSIGNED: This study suggests that AMFR could be used as a therapeutic strategy for the clinical treatment of glioblastoma.