■蜱传病毒(TBV)对人类和其他脊椎动物的健康构成重大风险。一类多节段类黄酮病毒,荆门蜱病毒(JMTV)最早是在湖北荆门采集的小毛蜱中发现的,2010年中国。已经证实JMTV在载体和宿主中具有相对广泛的分布,并且与人类疾病有关。
■在卧龙自然保护区收集了寄生和寻找寄主的蜱虫,四川省。提取总RNA,然后富集病毒RNA。构建DNA文库,然后用MGI高通量测序组(PE150)测序。在衔接子序列之后,去除了低质量的碱基和宿主基因组,分类为病毒的结果读数随后从头组装成重叠群,然后将其与NT数据库进行比较。在王国病毒下注释的那些最初被鉴定为潜在的病毒相关序列。使用MEGA和SimPlot软件进行序列的系统发育和重排分析,分别。
■收集了以大熊猫和山羊为食的两个寻求寄主的蜱和17个蜱。通过高通量测序,从4个蜱虫样本(PC-13,PC-16,PC-18和PC-19)获得了完整的病毒基因组,这些样本与已知的JMTV具有88.7-96.3%的相似性.系统发育树表明这是一种新型的JMTV样病毒,被称为四川蜱病毒,也有与其他JMTV菌株重组的信号,提示在多个蜱宿主之间存在分段黄样病毒的跨物种传播和共感染。
■我们发现并确认了一种新的荆门蜱病毒,四川蜱病毒。需要进一步调查以确定四川蜱病毒对人和动物的致病性,以及其在自然界中的流行病学特征。
UNASSIGNED: Tick-borne viruses (TBVs) pose a significant risk to the health of humans and other vertebrates. A class of multisegmented flavi-like viruses, Jingmen tick virus (JMTV) was first discovered in Rhipicephalus microplus ticks collected from Jingmen of Hubei Province, China in 2010. JMTV has been confirmed to have a relatively wide distribution in vectors and hosts and is associated with human diseases.
UNASSIGNED: Parasitic and host-seeking ticks were collected in Wolong Nature Reserve, Sichuan Province. Total RNA was extracted and then enriched the viral RNA. The DNA library was constructed and then were sequenced with MGI High-throughput Sequencing Set (PE150). After the adaptor sequences,low-quality bases and host genome were removed, resulting reads classified as a virus were subsequently de novo assembled into contigs, which were then compared to the NT database. Those annotated under the kingdom virus were initially identified as potential virus-associated sequences. Phylogenetic and Reassortment analysis of sequences were performed using MEGA and SimPlot software, respectively.
UNASSIGNED: Two host-seeking ticks and 17 ticks that fed on giant pandas and goats were collected. Through high-throughput sequencing, whole virus genomes were attained from four tick samples (PC-13, PC-16, PC-18, and PC-19) that shared 88.7-96.3% similarity with known JMTV. Phylogenetic tree showed that it was a novel JMTV-like virus, referred to as Sichuan tick virus, which also had the signals of reassortment with other JMTV strains, suggesting a cross-species transmission and co-infection of segmented flavi-like viruses among multiple tick hosts.
UNASSIGNED: We discovered and confirmed one new Jingmen tick virus, Sichuan tick virus. Further investigation is required to determine the pathogenicity of Sichuan tick virus to humans and animals, as well as its epidemiological characteristics in nature.