背景:自闭症谱系障碍是一种发育障碍,可以影响自闭症的丙戊酸(Val)啮齿动物模型的感觉运动行为。尽管晶须剥夺(WD)会引起皮质神经元的可塑性变化,在新生儿期的触觉刺激(TS)可能会逆转它。这里,我们研究了TS和WD对Val治疗的青少年桶状皮层神经元行为和组织学特征的相互作用。
方法:对照(CTL,CTL-TS,CTL-WD,和CTL-TS-WD组)和Val治疗(Val,Val-TS,Val-WD,和Val-TS-WD组)对男女大鼠进行社交互动的行为测试,和新颖的纹理识别,和Nissl染色。TS组暴露于感觉刺激15分钟,三次/天;此外,WD组的所有晶须从出生后第1天至第21天每隔一天修剪一次.
结果:产前丙戊酸给药和出生后WD均降低了男女Val和CTL-WD组在社交互动和质地辨别测试中与CTL组相比的大鼠表现百分比。在TS之后,与Val组相比,Val-TS-WD组的性能显着提高(p<0.05),而CTL-TS-WD组的表现拯救了CTL组。Nissl染色结果还显示,与CTL组相比,Val和CTL-WD组的桶视野区神经元退变百分比显着增加(p<0.05)。在这方面,TS降低了Val-TS-WD和CTL-TS-WD组的神经元变性百分比,与CTL组相比,显著(p<0.05)。
结论:TS在幼年雄性和雌性大鼠中可以充当调节剂,并补偿WD和产前丙戊酸暴露的行为和组织学后果。
Autism spectrum disorder is a developmental disorder that can affect sensory-motor behaviors in the valproic acid (Val) rodent model of autism. Although whisker deprivation (WD) induces plastic changes in the cortical neurons, tactile stimulation (TS) during the neonatal period may reverse it. Here, we investigate the interaction effects of TS and WD on behavioral and histologic features of barrel cortex neurons in juvenile Val-treated.
Control (CTL, CTL-TS, CTL-WD, and CTL-TS-WD groups) and Val-treated (Val, Val-TS, Val-WD, and Val-TS-WD groups) rats of both sexes were subjected to behavioral tests of social interaction, and novel texture recognition, and Nissl staining. The TS groups were exposed to sensory stimulation for 15 min, three times/day; moreover, all whiskers in the WD groups were trimmed every other day from postnatal days 1 to 21.
Both prenatal valproic acid administration and postnatal WD decreased the rats\' performance percentage of the Val and CTL-WD groups of both sexes compared with the CTL groups in the social interaction and texture discrimination tests. Following TS, the performance of the Val-TS-WD group increased significantly compared to the Val group (p < .05), whereas the performance of the CTL-TS-WD group rescued to the CTL group. Nissl staining results also revealed the neuron degeneration percentage in the barrel field area of the Val and CTL-WD groups was increased significantly (p < .05) compared with the CTL group. In this regard, TS decreased the neuron degeneration percentage of the Val-TS-WD and the CTL-TS-WD groups, compared with the CTL group, significantly (p < .05).
TS in juvenile male and female rats can act as a modulator and compensate for the behavioral and histological consequences of WD and prenatal valproic acid exposure.