在这个项目中,合成了三维氧化石墨烯包覆的琼脂糖/壳聚糖(ACGO)多孔膜,并将其用作薄膜微萃取(TFME)技术中的吸附剂,2,4-二氯苯酚,3,5-二氯苯酚和2,4,6-三氯苯酚作为农业废水等各种实际样品中的模型分析物,蜂蜜和茶样品。此外,以四乙基氯化铵/氯化氯组成的低共熔溶剂作为解吸溶剂。各种变量的影响,如:提取时间,搅拌速率,溶剂解吸体积,解吸时间,对离子强度和溶液pH值对萃取效率的影响进行了研究和优化。在优化条件下,该方法的线性范围为0.1-500μgL-1,用于测试分析物(4-氯酚=0.1-500μgL-1,2,4-二氯苯酚=0.2-500μgL-1,3,5-二氯苯酚=0.5-500μgL-1和2,4,6-三氯苯酚=0.2-500μgL-1)。获得的相关系数(r2)在0.9984和0.9994之间。检测限(LODs)也在0.03-0.13μgL-1之间计算。获得的相对标准偏差(RSDs%)在2.8至5.9%的范围内。所研究分析物的富集因子(EF)值也在33.4-35.8的范围内。此外,结果表明,所制备的薄膜有可能用于环境领域的更多应用,食品安全,和药物分析。
In this project, a three-dimensional graphene oxide coated agarose/chitosan (ACGO) porous film was synthesized and utilized as sorbent in thin film microextraction (TFME) technique to extract 4-chlorophenol, 2,4-dichlorophenol, 3,5-dichlorophenol and 2,4,6-trichlorophenol as the model analytes in various real samples such as agricultural waste water, honey and tea samples. In addition, deep eutectic solvent made of tetra ethyl ammonium chloride/chlorine chloride was used as a desorption solvent. The effect of various variables, such as: extraction time, stirring rate, solvent desorption volume, desorption time, ionic strength and solution pH on the extraction efficiency of the method was studied and optimized. Under the optimized condition, the linear range of the method was obtained in the range of 0.1-500μgL-1 for testing analytes (4-chloropheol=0.1-500μgL-1, 2,4-dichlorophenol=0.2-500μgL-1, 3,5-dichlorophenol=0.5-500μgL-1 and 2,4,6-trichlorophenol=0.2-500μgL-1). The obtained correlation coefficients (r2) were between 0.9984 and 0.9994. The limits of detection (LODs) were also calculated between 0.03 - 0.13μgL-1. The relative standard deviations (RSDs%) were obtained in the range of 2.8 to 5.9%. The enrichment factor (EFs) values for the studied analytes were also obtained in the range of 33.4-35.8. In addition, the obtained results indicated that the prepared film can potentially be used for more applications in the field of environment, food safety, and drug analysis.