报告使用Eusol-C冷冻角膜作为构造角膜移植物的临床结果。回顾性回顾2013年至2020年接受冰冻角膜急诊构造移植物患者的医疗记录。角膜已在-78°C的Eusol-C保存培养基中储存了6.9个月的平均时间。诊断,移植特点,微生物培养结果,解剖完整性,上皮愈合,新生血管形成,透明度,感染和需要额外的手术登记。40例患者使用了50个角膜(平均年龄60.5岁,20名男性),术后中位随访时间为27.3个月。需要构造移植物是由于:穿孔继发于免疫疾病(6,12%),神经营养性溃疡(11,22%),创伤(3,6%),角膜感染(11,22%),眼表慢性疾病(9,18%)和先前的角膜移植失败(10,22%)。移植物的平均大小为5.6mm,36例(72%)也接受了羊膜移植物。38个角膜实现了上皮化(76%),25(50%)清晰,19(38%)发展了新血管形成。没有一个角膜被拒绝。17个角膜(34%)失败:7个(14%)由于基线疾病的重新激活,10个(20%)由于原发性移植物失败。四个角膜(8%)的微生物培养物呈阳性,提示污染,两个(4%)的角膜脓肿与微生物培养物呈阳性无关。在-78°C下在Eusol-C中长期保存供体角膜是一种可行的技术,可以用最少的设备满足紧急移植物的需求。
To report the clinical results on the use of corneas frozen in Eusol-C as tectonic corneal grafts.Retrospective review of medical records of patients who received frozen corneas as emergency tectonic grafts from 2013 to 2020. Corneas had been stored in Eusol-C preservation media at - 78 °C for a mean time of 6.9 months. Diagnosis, transplant characteristics, microbial culture results, anatomic integrity, epithelial healing, neovascularization, transparency, infection and need for additional surgeries were registered. Fifty corneas were used in 40 patients (mean age 60.5 years, 20 males) with a median follow-up of 27.3 months after surgery. Need for tectonic graft was due to: perforation secondary to immune diseases (6, 12%), neurotrophic ulcer (11, 22%), trauma (3, 6%), corneal infection (11, 22%), chronic disorders of the ocular surface (9, 18%) and previous corneal graft failure (10, 22%). Mean size of grafts was 5.6 mm and 36 cases (72%) also received an amniotic membrane graft. Thirty-eight corneas achieved epithelization (76%), 25 (50%) were clear and 19 (38%) developed neovascularization. None of the corneas were rejected. Seventeen corneas (34%) failed: 7 (14%) due to reactivation of baseline disease and 10 (20%) due to primary graft failure. Four corneas (8%) had positive microbial cultures suggestive of contamination and 2 (4%) developed a cornea abscess non-related to a positive microbial culture. Long-term preservation of donor corneas in Eusol-C at - 78 °C is a viable technique to meet the needs of emergency grafts with minimal equipment.