胡萝卜科(Apiaceae)包括466属,其中包括许多著名的作物(例如,茴香,caraway,胡萝卜,芹菜,香菜,孜然,dill,茴香,欧芹,和欧洲防风草)。亚科之间的高级系统发育关系,部落,和其他主要的矢车菊科没有完全解决。本研究旨在解决这一重要的知识差距。
使用通用Angiosperms353探针集捕获目标序列,以检查234个天麻属的系统发育关系,代表所有四个目前公认的亚家族(Apioideae,亚速藻,Mackinlayoideae,和Saniculoideae)。使用多物种聚结和串联方法分析恢复的核基因。
我们甚至从古老而劣质的植物标本室标本中回收了数百个核基因。特别值得注意的是,我们在大力支持下放置了三个incertaesedis属(Platysace,Klotzchia,和Hermas);所有三个人都占据孤立的位置,与Platysace决定成为所有剩余的伞形科的姐妹。我们放置了九个属(Apodicarpum,Bonannia,Grafia,单倍体,Microsciavium,Physotrichia,产前检查,Tricholaser,Xatardia)以前从未被包括在任何分子系统发育研究中。
我们提供支持,以维持现有的四个木科的亚科,在认识到Hermas的同时,Klotzschia,并且Platysace进化枝可能每个都需要容纳在其他子家庭中(有待改进的采样)。可以通过扩展Saniculoideae亚科来适应当前的Apioid属的放置,尽管尚未定义此分组的足够形态突触。这是第一个使用高通量测序方法对伞形科进行的系统发育研究,代表了该群体前所未有的进化框架。
The carrot family (Apiaceae) comprises 466 genera, which include many well-known crops (e.g., aniseed, caraway, carrots, celery, coriander, cumin, dill, fennel, parsley, and parsnips). Higher-level phylogenetic relationships among subfamilies, tribes, and other major clades of Apiaceae are not fully resolved. This study aims to address this important knowledge gap.
Target sequence capture with the universal Angiosperms353 probe set was used to examine phylogenetic relationships in 234 genera of Apiaceae, representing all four currently recognized subfamilies (Apioideae, Azorelloideae, Mackinlayoideae, and Saniculoideae). Recovered nuclear genes were analyzed using both multispecies coalescent and concatenation approaches.
We recovered hundreds of nuclear genes even from old and poor-quality herbarium specimens. Of particular note, we placed with strong support three incertae sedis genera (Platysace, Klotzchia, and Hermas); all three occupy isolated positions, with Platysace resolved as sister to all remaining Apiaceae. We placed nine genera (Apodicarpum, Bonannia, Grafia, Haplosciadium, Microsciadium, Physotrichia, Ptychotis, Tricholaser, Xatardia) that have never previously been included in any molecular phylogenetic study.
We provide support for the maintenance of the four existing subfamilies of Apiaceae, while recognizing that Hermas, Klotzschia, and the Platysace clade may each need to be accommodated in additional subfamilies (pending improved sampling). The placement of the currently apioid genus Phlyctidocarpa can be accommodated by the expansion of subfamily Saniculoideae, although adequate morphological synapomorphies for this grouping are yet to be defined. This is the first phylogenetic study of the Apiaceae using high-throughput sequencing methods and represents an unprecedented evolutionary framework for the group.