作为一种常见的食源性致病菌,单核细胞增生李斯特菌感染对人类生命和健康构成重大威胁。这项研究的目的是利用比较基因组学来揭示来自不同地区的单核细胞增生李斯特菌菌株的生物多样性和进化特征。筛选潜在的靶基因和挖掘新的靶基因,从而为单增李斯特菌菌株的特异性分子检测和治疗靶点提供了重要的参考价值。全基因组分析表明,来自不同地区的单核细胞增生李斯特菌具有开放的基因组,为适应不同的环境提供了坚实的遗传基础。这些菌株含有许多毒力基因,这些基因有助于它们的高致病性。它们还表现出相对较高的膦酸抗性,糖肽,lincosamide,和肽抗生素。移动遗传元件的结果表明,尽管位于不同的地理位置,在细菌基因组进化和适应特定环境压力方面存在一定程度的相似性。通过泛基因组学鉴定的潜在靶基因主要与单核细胞增生李斯特菌的基本生命活动和感染侵袭有关,包括已知的目标,如inlB,可用于分子检测和治疗目的。在筛选了大量潜在的靶基因后,我们使用hub基因选择方法进一步筛选它们,以挖掘新的靶基因。本研究采用了八种不同的枢纽基因筛选方法,最终确定十个高度连接的集线器基因(bglF_1,davD,menE_1,tils,dapx,iolC,gshAB,cysG,trpA,andhISC),在单核细胞增生李斯特菌的发病机制中起着至关重要的作用。全基因组分析结果表明,来自不同地区的单核细胞增生李斯特菌在细菌基因组进化中表现出高度相似性。PCR结果表明bglF_1和davD基因对单核细胞增生李斯特菌具有优异的特异性。因此,bglF_1和davD基因有望作为来自不同地区的单核细胞增生李斯特菌菌株的特异性分子检测和治疗靶标。
As a common foodborne pathogen, infection with L. monocytogenes poses a significant threat to human life and health. The objective of this study was to employ comparative genomics to unveil the biodiversity and evolutionary characteristics of L. monocytogenes strains from different regions, screening for potential target genes and mining novel target genes, thus providing significant reference value for the specific molecular detection and therapeutic targets of L. monocytogenes strains. Pan-genomic analysis revealed that L. monocytogenes from different regions have open genomes, providing a solid genetic basis for adaptation to different environments. These strains contain numerous virulence genes that contribute to their high pathogenicity. They also exhibit relatively high resistance to phosphonic acid, glycopeptide, lincosamide, and peptide antibiotics. The results of mobile genetic elements indicate that, despite being located in different geographical locations, there is a certain degree of similarity in bacterial genome evolution and adaptation to specific environmental pressures. The potential target genes identified through pan-genomics are primarily associated with the fundamental life activities and infection invasion of L. monocytogenes, including known targets such as inlB, which can be utilized for molecular detection and therapeutic purposes. After screening a large number of potential target genes, we further screened them using hub gene selection methods to mining novel target genes. The present study employed eight different hub gene screening methods, ultimately identifying ten highly connected hub genes (bglF_1, davD, menE_1, tilS, dapX, iolC, gshAB, cysG, trpA, and hisC), which play crucial roles in the pathogenesis of L. monocytogenes. The results of pan-genomic analysis showed that L. monocytogenes from different regions exhibit high similarity in bacterial genome evolution. The PCR results demonstrated the excellent specificity of the bglF_1 and davD genes for L. monocytogenes. Therefore, the bglF_1 and davD genes hold promise as specific molecular detection and therapeutic targets for L. monocytogenes strains from different regions.