温度升高会影响各种生物的栖息地,包括微观无脊椎动物。为了深入了解缓行随温度的变化,我们分离了暴露在不同温度下的储存细胞,并在活性和tun状态的ParamacrobiotusexperimentalisKaczmarek中进行了生化和超微结构分析,Mioduchowska,Poprawa,&Roszkowska,2020年。在不同温度(20°C,30°C,35°C,37°C,40°C,和42°C)在从Pam的活性标本中分离的存储单元中。实验者。在活跃的动物中,随着外部温度的升高,我们观察到HSPs(HSP27,HSP60和HSP70)水平升高.此外,随着温度的升高,储存细胞超微结构变化的数量增加。细胞器,如线粒体和粗面内质网,逐渐退化。在42°C时,细胞死亡发生坏死。除了核质的较高电子密度和某些线粒体中电子致密物质的积累(在42°C时)之外,暴露于不同温度的tun储存细胞的超微结构几乎没有变化。我们得出的结论是干燥的(tun-state)耐高温,但不是活跃的缓步(再水化24小时后的金枪鱼存活率:20℃时为93.3%,35°C时为60.0%,37°C时为33.3%,在40°C时为33.3%,和20.0%在42°C)。
Increasing temperature influences the habitats of various organisms, including microscopic invertebrates. To gain insight into temperature-dependent changes in
tardigrades, we isolated storage cells exposed to various temperatures and conducted biochemical and ultrastructural analysis in active and tun-state Paramacrobiotus experimentalis Kaczmarek, Mioduchowska, Poprawa, & Roszkowska, 2020. The abundance of heat shock proteins (HSPs) and ultrastructure of the storage cells were examined at different temperatures (20 °C, 30 °C, 35 °C, 37 °C, 40 °C, and 42 °C) in storage cells isolated from active specimens of Pam. experimentalis. In the active animals, upon increase in external temperature, we observed an increase in the levels of HSPs (HSP27, HSP60, and HSP70). Furthermore, the number of ultrastructural changes in storage cells increased with increasing temperature. Cellular organelles, such as mitochondria and the rough endoplasmic reticulum, gradually degenerated. At 42 °C, cell death occurred by necrosis. Apart from the higher electron density of the karyoplasm and the accumulation of electron-dense material in some mitochondria (at 42 °C), almost no changes were observed in the ultrastructure of tun storage cells exposed to different temperatures. We concluded that desiccated (tun-state) are resistant to high temperatures, but not active
tardigrades (survival rates of tuns after 24 h of rehydration: 93.3% at 20 °C, 60.0% at 35 °C, 33.3% at 37 °C, 33.3% at 40 °C, and 20.0% at 42 °C).