嗜麦芽窄食单胞菌被发现是与根际相关的土壤细菌。稍后,研究发现嗜麦芽窄食链球菌是一种多重耐药的医院相关病原体。裂解噬菌体是潜在的抗微生物剂;因此,有必要分离和鉴定新的寡养单胞菌噬菌体。噬菌体StenM_174是从家禽养殖场的凋落物中分离的,使用嗜麦芽嗜血杆菌的临床菌株作为宿主。StenM_174在广泛的临床和环境菌株中繁殖,主要是嗜麦芽链球菌,它有podovirus形态。StenM_174基因组序列长度为42,956bp,它包含52个推定的基因。所有基因都是单向的,其中31个编码具有预测功能的蛋白质,而其余21人被确定为假设。使用AlphaFold2结构模型预测了StenM_174的两个尾部刺突蛋白。对基因组的比较分析表明,窄食单胞菌噬菌体StenM_174与噬菌体Ponderosa,Pepon,Ptah,和TS-10,可以是自拟病毒科中新推定的黄体病毒属的成员。此外,分析的数据表明该家族中有一个新的亚家族。
Stenotrophomonas maltophilia was discovered as a soil bacterium associated with the rhizosphere. Later, S. maltophilia was found to be a multidrug-resistant hospital-associated pathogen. Lytic bacteriophages are prospective antimicrobials; therefore, there is a need for the isolation and characterization of new Stenotrophomonas phages. The phage StenM_174 was isolated from litter at a poultry farm using a clinical strain of S. maltophilia as the host. StenM_174 reproduced in a wide range of clinical and environmental strains of Stenotrophomonas, mainly S. maltophilia, and it had a podovirus morphotype. The length of the genomic sequence of StenM_174 was 42,956 bp, and it contained 52 putative genes. All genes were unidirectional, and 31 of them encoded proteins with predicted functions, while the remaining 21 were identified as hypothetical ones. Two tail spike proteins of StenM_174 were predicted using AlphaFold2 structural modeling. A comparative analysis of the genome shows that the Stenotrophomonas phage StenM_174, along with the phages Ponderosa, Pepon, Ptah, and TS-10, can be members of the new putative genus Ponderosavirus in the Autographiviridae family. In addition, the analyzed data suggest a new subfamily within this family.