■泛素化是一种重要的翻译后修饰。然而,尚未确定E3泛素连接酶的TRIM家族在头颈部鳞状细胞癌(HNSCC)中的意义。在这项研究中,评估了TRIME3泛素连接酶在头颈部鳞状细胞癌(HNSCC)淋巴管浸润中的作用。
■TRIM表达式和相关参数从UbiBrowser2.0,UALCAN,TIMER,TISIB,LinkedOmics,STRING,和GeneMANIA数据库。免疫组织化学用于确认它们的表达。
■TRIM2、TRIM11、TRIM28和TRIM56在伴淋巴管浸润的HNSCC中上调。TRIM表达与免疫浸润密切相关,包括关键的治疗目标,如PD-1和CTL4。共表达的基因和可能的泛素化底物包括肿瘤相关因子。TRIM在泛素化相关通路和重要信号通路中具有预测作用,例如,MAPK,PI3K-Akt,和JAK-STAT信号通路。
■四种TRIM介导的泛素化可能参与肿瘤免疫的调节,为未来研究TRIM家族在HNSCC预测和个性化医疗中的作用奠定基础。四种TRIM可能通过促进HNSCC的淋巴管浸润而发挥致癌作用。
UNASSIGNED: Ubiquitination is an important post-translational modification. However, the significance of the
TRIM family of E3 ubiquitin ligases in head and neck squamous cell carcinoma (HNSCC) has not been determined. In this study, the roles of
TRIM E3 ubiquitin ligases in lymphovascular invasion in head and neck squamous cell carcinoma (HNSCC) were evaluated.
UNASSIGNED: TRIM expression and related parameters were obtained from UbiBrowser2.0, UALCAN, TIMER, TISIDB, LinkedOmics, STRING, and GeneMANIA databases. Immunohistochemistry was used to confirm their expression.
UNASSIGNED: TRIM2, TRIM11, TRIM28, and TRIM56 were upregulated in HNSCC with lymphovascular invasion.
TRIM expression was strongly associated with immune infiltration, including key treatment targets, like PD-1 and CTL4. Co-expressed genes and possible ubiquitination substrates included tumor-related factors. The TRIMs had predicted roles in ubiquitination-related pathways and vital signaling pathways, eg, MAPK, PI3K-Akt, and JAK-STAT signaling pathways.
UNASSIGNED: Ubiquitination mediated by four TRIMs might be involved in the regulation of tumor immunity, laying the foundation for future studies of the roles of the
TRIM family on the prediction and personalized medicine in HNSCC. The four TRIMs might exert oncogenic effects by promoting lymphovascular invasion in HNSCC.