未经授权:口腔鳞状细胞癌(OSCC)是全球最常见的恶性肿瘤之一。最近,免疫检查点抑制剂的施用为癌症治疗开辟了更多的可能性。
UNASSIGNED:我们在TCGA数据库中使用了加权基因共表达网络和单样本基因集富集分析(ssGSEA)算法,并确定了与调节性T细胞(Treg)丰度高度相关的模块。随后,我们通过组织微阵列验证了结果,并利用免疫组织化学染色(IHC)检验了表达水平与临床病理分期之间的关系.CCK-8Transwell,和伤口愈合试验用于检测OSCC细胞的功能。
未经批准:LCK,IL10RA,选择TNFRSF1B作为与调节性T细胞浸润相关的生物标志物。IHC染色显示LCK表达明显增高,OSCC患者的IL10RA或TNFRSF1B,表达水平与肿瘤分期有关,淋巴结转移,病理阶段,临床状态和总生存期。体外实验表明,LCK,IL10RA或TNFRSF1B敲低有效地损害了增殖,迁徙,和OSCC细胞系的侵袭能力。
UNASSIGNED:我们在OSCC中进行了一系列生物信息学分析,并确定了三个致癌指标:LCK,IL10RA,TNFRSF1B。这些发现揭示了hub基因的潜在预后价值,从而为OSCC的深入研究奠定基础。
UNASSIGNED: Oral squamous cell carcinoma (OSCC) is one of the most prevalent malignancies worldwide. More recently, the administration of immune checkpoint inhibitors has opened up more possibilities for cancer treatment.
UNASSIGNED: We utilized a weighted gene co-expression network and the single sample gene set enrichment analysis (ssGSEA) algorithm in the TCGA database and identified a module highly correlated with regulatory T cell (Treg) abundance in OSCC. Subsequently, we verified the results by tissue microarrays and utilized immunohistochemical staining (IHC) to test the relationship between the expression level and clinicopathological staging. CCK-8, transwell, and wound healing assays were utilized to detect the functions of OSCC cells.
UNASSIGNED: LCK, IL10RA, and
TNFRSF1B were selected as biomarkers related to regulatory T cell infiltration. IHC staining showed significantly increased expression of LCK, IL10RA or
TNFRSF1B in OSCC patients, and the expression levels were associated with tumor stage, lymph node metastasis, pathological stage, clinical status and the overall survival. In vitro experiments showed that LCK, IL10RA or
TNFRSF1B knockdown efficiently impaired the proliferative, migrative, and invasive capacity in OSCC cell lines.
UNASSIGNED: We performed a series of bioinformatics analyses in OSCC and identified three oncogenic indicators: LCK, IL10RA,
TNFRSF1B. These findings uncovered the potential prognostic values of hub genes, thus laying foundations for in-depth research in OSCC.