EB病毒(EBV)与各种类型的人类恶性肿瘤有关,包括鼻咽癌(NPC),EBV相关胃癌(EBVaGC),口腔鳞状细胞癌(OSCC)。本研究旨在通过对细胞系和肿瘤组织进行综合生物信息学分析,确定可用于预测EBV相关上皮癌(EBVaCAs)预后的基因特征和常见信号通路。我们在EBVaCA细胞系中鉴定了12个差异表达基因(DEGs)。其中,只有四个DEG,包括巴比,SLC26A9、SGPP2和TMC8显著上调。然而,与EBV阴性肿瘤组织相比,SLC26A9和TMC8,而不是BAMBI和SGPP2,在EBV阳性肿瘤组织中显著上调。接下来,我们确定IL6/JAK/STAT3和TNF-α/NF-κB信号通路是EBVaCAs的常见标志。与这两个标志相关的关键基因的表达在EBV感染的细胞系和EBV阳性肿瘤组织中均上调。这些结果表明,SLC26A9和TMC8可能是可以有效预测EBVaCAs预后的基因特征,并为EBV驱动的上皮癌的分子机制提供了新的见解。
Epstein-Barr virus (EBV) is associated with various types of human malignancies, including nasopharyngeal carcinoma (NPC), EBV-associated gastric carcinoma (EBVaGC), and oral squamous cell carcinoma (OSCC). The present study aimed to identify gene signatures and common signaling pathways that can be used to predict the prognosis of EBV-associated epithelial cancers (EBVaCAs) by performing an integrated bioinformatics analysis of cell lines and tumor tissues. We identified 12 differentially expressed genes (DEGs) in the EBVaCA cell lines. Among them, only four DEGs, including BAMBI, SLC26A9, SGPP2, and
TMC8, were significantly upregulated. However, SLC26A9 and
TMC8, but not BAMBI and SGPP2, were significantly upregulated in EBV-positive tumor tissues compared to EBV-negative tumor tissues. Next, we identified IL6/JAK/STAT3 and TNF-α/NF-κB signaling pathways as common hallmarks of EBVaCAs. The expression of key genes related to the two hallmarks was upregulated in both EBV-infected cell lines and EBV-positive tumor tissues. These results suggest that SLC26A9 and
TMC8 might be gene signatures that can effectively predict the prognosis of EBVaCAs and provide new insights into the molecular mechanisms of EBV-driven epithelial cancers.