干扰素-γ(IFN-γ)是一种在免疫调节中起重要作用的细胞因子。特别是在免疫细胞的激活和分化中。Toll样受体(TLR)是一组模式识别受体,可感知与病原体相关的结构基序并提醒免疫细胞入侵。IFN-γ和TLR激动剂都已用作免疫佐剂以增强癌症免疫疗法和疫苗对传染病或精神活性化合物的功效。在这项研究中,我们旨在探讨IFN-γ和TLR激动剂同时应用以促进树突状细胞活化和随后的抗原呈递的潜力.简而言之,小鼠树突状细胞用IFN-γ和/或TLR激动剂处理,聚肌苷酸-聚胞嘧啶酸(聚I:C),或瑞喹莫特(R848)。接下来,对树突状细胞进行活化标记染色,分化簇86(CD86),通过流式细胞术测量CD86阳性细胞的百分比。从细胞计数分析来看,IFN-γ有效刺激相当数量的树突状细胞,而与对照相比,TLR激动剂本身只能激活一些。IFN-γ与聚I:C或R848的组合比单独的IFN-γ引发更大量的树突细胞活化。例如,10ng/mLIFN-γ与100µg/mL聚I:C实现了59.1%的细胞活化,显着高于10ng/mLIFN-γ获得的33.4%CD86阳性细胞。这些结果表明,IFN-γ和TLR激动剂可以作为互补系统来促进树突状细胞的活化和抗原呈递。这两类分子之间可能存在协同作用,但需要进一步调查,以确定他们的促进活动的相互作用。
Interferon-γ (IFN-γ) is a cytokine that plays an important role in immune regulation, especially in the activation and differentiation of immune cells. Toll-like receptors (TLRs) are a family of pattern-recognition receptors that sense structural motifs related to pathogens and alert immune cells to the invasion. Both IFN-γ and TLR agonists have been used as immunoadjuvants to augment the efficacy of cancer immunotherapies and vaccines against infectious diseases or psychoactive compounds. In this study, we aimed to explore the potential of IFN-γ and TLR agonists being applied simultaneously to boost dendritic cell activation and the subsequent antigen presentation. In brief, murine dendritic cells were treated with IFN-γ and/or the TLR agonists, polyinosinic-polycytidylic acid (poly I:C), or resiquimod (R848). Next, the dendritic cells were stained for an activation marker, a cluster of differentiation 86 (CD86), and the percentage of CD86-positive cells was measured by flow cytometry. From the cytometric analysis, IFN-γ efficiently stimulated a considerable number of the dendritic cells, while the TLR agonists by themselves could merely activate a few compared to the control. The combination of IFN-γ with poly I:C or R848 triggered a higher amount of dendritic cell activation than IFN-γ alone. For instance, 10 ng/mL IFN-γ with 100 µg/mL poly I:C achieved 59.1% cell activation, which was significantly higher than the 33.4% CD86-positive cells obtained by 10 ng/mL IFN-γ. These results suggested that IFN-γ and TLR agonists could be applied as complementary systems to promote dendritic cell activation and antigen presentation. There might be a synergy between the two classes of molecules, but further investigation is warranted to ascertain the interaction of their promotive activities.