未经批准:尖刺棒杆菌(C.accolens)是一种常见的鼻腔定植剂,而金黄色葡萄球菌(S.金黄色葡萄球菌)通常被认为是慢性鼻窦炎(CRS)患者的病原生物。本研讨旨在评价两种细菌在体外的互相感化。
未经授权:从鼻窦拭子中分离出的accolens梭菌和金黄色葡萄球菌,以及从健康和CRS患者的细胞刷洗中培养的原代人鼻上皮细胞(HNEC)用于本研究。测试了单独生长和共培养的所有分离株的无细胞培养上清液对跨上皮电阻(TER)的影响,FITC-葡聚糖渗透性,乳酸脱氢酶(LDH),以及HNECs分泌IL-6和IL-8。共聚焦扫描激光显微镜和免疫荧光也用于可视化顶端连接复合物。还测试了无细胞培养上清液的抗微生物活性和在浮游和生物膜金黄色葡萄球菌生长上的生长。
未经证实:3\\C的无细胞培养上清液accolens菌株(金黄色葡萄球菌参考菌株为60%,金黄色葡萄球菌临床菌株为30%浓度)显着抑制浮游金黄色葡萄球菌参考菌株和临床菌株的生长。无细胞培养上清液未引起HNEC-ALI培养物的TER或FITC-葡聚糖通透性变化,而金黄色葡萄球菌菌株的无细胞培养上清液具有不利影响。与金黄色葡萄球菌的临床和参考菌株共培养的无细胞培养上清液以剂量依赖性方式延迟了金黄色葡萄球菌依赖性粘膜屏障损伤。
未经证实:无细胞培养上清液似乎抑制金黄色葡萄球菌浮游细菌的生长,并可减少金黄色葡萄球菌引起的粘膜屏障损伤。
UNASSIGNED: Corynebacterium accolens (C. accolens) is a common nasal colonizer, whereas Staphylococcus aureus (S. aureus) is typically regarded a pathogenic organism in patients with chronic rhinosinusitis (CRS). This study aims to evaluate the interaction of the two bacteria in vitro.
UNASSIGNED: Clinical isolates of C. accolens and S. aureus from sinonasal swabs, as well as primary human nasal epithelial cells (HNECs) cultured from cellular brushings of both healthy and CRS patients were used for this study. The cell-free culture supernatants of all isolates grown alone and in co-cultures were tested for their effects on transepithelial electrical resistance (
TER), FITC-Dextran permeability, lactate dehydrogenase (LDH), and IL-6 and IL-8 secretion of HNECs. Confocal scanning laser microscopy and immunofluorescence were also used to visualize the apical junctional complexes. C. accolens cell-free culture supernatants were also tested for antimicrobial activity and growth on planktonic and biofilm S. aureus growth.
UNASSIGNED: The cell-free culture supernatants of 3\\C. accolens strains (at 60% for S. aureus reference strain and 30% concentration for S. aureus clinical strains) inhibited the growth of both the planktonic S. aureus reference and clinical strains significantly. The C. accolens cell-free culture supernatants caused no change in the
TER or FITC-Dextran permeability of the HNEC-ALI cultures, while the cell-free culture supernatants of S. aureus strains had a detrimental effect. Cell-free culture supernatants of C. accolens co-cultured with both the clinical and reference strains of S. aureus delayed the S. aureus-dependent mucosal barrier damage in a dose-dependent manner.
UNASSIGNED: Corynebacterium accolens cell-free culture supernatants appear to inhibit the growth of the S. aureus planktonic bacteria, and may reduce the mucosal barrier damage caused by S. aureus.