急性肝损伤,有一种危险的神经系统疾病称为肝性脑病(HE)。Herbacetin是一种具有许多药理特征的糖基化黄酮类化合物。这项研究的目的是评估除草剂抗与硫代乙酰胺(TAA)大鼠模型相关的认知缺陷的能力,并描述潜在的行为和药理机制。大鼠用草地辛(20和40mg/kg)预处理30天。第30天,以单剂量给大鼠注射TAA(i.p.350mg/kg)。除了组织学研究,大脑的超结构结构,肝功能,氧化应激生物标志物,和行为测试进行了评估。与TAA中毒组相比,除草剂改善了运动和认知缺陷,血清肝毒性指数和氨水平。Herbacetin降低了Malodialdeyde的大脑水平,谷氨酰胺合成酶(GS),肿瘤坏死因子-α(TNF-α),白细胞介素1B(IL-1β),附件五,增加大脑GSH,Sirtuin1(SIRT1),和AMP激活的激酶(AMPK)表达水平。此外,除草剂通过减少炎症细胞和凋亡细胞的数量来改善脑组织的组织病理学改变和超微结构。Herbacetin处理显著降低了由TAA引起的毒性。这些发现表明,由于其减少氧化应激的能力,草皮素可能被认为是一种可能的神经保护和认知增强剂。与TAA相关的炎症和凋亡。
Acute liver injury, there is a risky neurological condition known as hepatic encephalopathy (HE). Herbacetin is a glycosylated flavonoid with many pharmacological characteristics. The purpose of this study was to assess the ability of herbacetin to protect against the cognitive deficits associated with thioacetamide (
TAA) rat model and delineate the underlying behavioral and pharmacological mechanisms. Rats were pretreated with herbacetin (20 and 40 mg/kg) for 30days. On 30th day, the rats were injected with
TAA (i.p. 350 mg/kg) in a single dose. In addition to a histpathological studies, ultra-structural architecture of the brain, liver functions, oxidative stress biomarkers, and behavioral tests were evaluated. Compared to the
TAA-intoxicated group, herbacetin improved the locomotor and cognitive deficits, serum hepatotoxicity indices and ammonia levels. Herbacetin reduced brain levels of malodialdeyde, glutamine synthetase (GS), tumor necrosis factor- alpha (TNF-α), interleukin 1 B (IL-1β), annexin v, and increased brain GSH, Sirtuin 1 (SIRT1), and AMP-activated kinase (AMPK) expression levels. Also, herbacetin improve the histopathological changes and ultra- structure of brain tissue via attenuating the number of inflammatory and apoptotic cells. Herbacetin treatment significantly reduced the toxicity caused by
TAA. These findings suggest that herbacetin might be taken into account as a possible neuroprotective and cognitive enhancing agent due to its ability to reduce oxidative stress, inflammation and apoptosis associated with
TAA.