三种新的木脂素糖苷,即,justisecundosidesA(1),B(2a),和C(2b),与七个已知化合物(3-9)一起从Justiciasecunda的整个植物中分离。它们的结构是基于HR-ESI-MS的综合分析建立的,IR,UV,CD,结合他们的1D和2D-NMR数据。化合物1-2a的推定生物遗传途径,b从针叶醇提出。此外,提取物的抗菌活性,分数,并对一些分离的化合物进行了抗多重耐药细菌和真菌菌株的评估。此外,抗疟药,Antileishmanial,并针对恶性疟原虫的敏感(3D7)和多药耐药(Dd2)菌株评估了抗锥虫活性,多诺瓦尼乳杆菌和血液形式,和布鲁氏锥虫,分别。化合物4对金黄色葡萄球菌SARN46003表现出中等的抗菌活性,MIC值为62.5μg/mL。此外,化合物6对恶性疟原虫的敏感(IC50Pf3D7:0.81μg/mL)和多药耐药(IC50PfDd2:14.61μg/mL)菌株表现出非常好的活性,而化合物4表现出良好的抗锥虫活性(IC50:1.19μg/mL)。此外,化合物1对多诺瓦尼乳杆菌的前鞭毛形式最活跃,IC50为13.02μg/mL。
Three new lignan glucosides, namely, justisecundosides A (1), B (2a), and C (2b), were isolated from the whole plant of Justicia secunda together with seven known compounds (3-9). Their structures were established based on a comprehensive analysis of HR-ESI-MS, IR, UV, and CD, in conjunction with their 1D and 2D-NMR data. A putative biogenetic pathway of compounds 1-2a,b from coniferyl alcohol was proposed. In addition, the antimicrobialactivities of the extract, fractions, and some isolated compounds were assessed against multiresistant bacterial and fungal strains. Furthermore, the antiplasmodial, antileishmanial, and antitrypanosomal activities were assessed against the sensitive (3D7) and multidrug-resistant (Dd2) strains of P. falciparum, promastigote and bloodstream forms of L. donovani, and Trypanosoma brucei, respectively. Compound 4 exhibited moderate antibacterial activity against Staphylococcus aureus SA RN 46003 with a MIC value of 62.5 μg/mL. Besides, compound 6 demonstrated a very good activity against sensitive (IC50Pf3D7: 0.81 μg/mL) and multidrug-resistant (IC50PfDd2: 14.61 μg/mL) strains of P. falciparum while compound 4 displayed good antitrypanosomal activity (IC50: 1.19 μg/mL). Also, compound 1 was the most active on the promastigote form of L. donovani with an IC50 of 13.02 μg/mL.