背景:评估T-bet和IFN-γ在下唇(LLSCC)和口腔舌鳞状细胞癌(OTSCC)中的免疫组织化学表达,验证Th1反应在不同临床条件下的病变中的存在。
方法:采用免疫组织化学方法对30例OTSCC和30例LLSCC进行分析。T-bet通过实质细胞和基质定量进行定量评估,和IFN-γ进行半定量分析:1:0-25%;2:26-50%;3:51-75%;4:>75%免疫阳性细胞。组织学分化程度分类为分化良好(WD),中度分化(MD),或分化差(PD)。
结果:OTSCC的T-bet+数量最高,实质(p:0.006),基质(p:0.156),实质/基质(p:0.015),与组织学恶性程度无关。IFN-γ在LLSCC中较高的浓度在实质中检测到,基质和实质/基质(p:0.000),以及在WD和MD中更高的免疫反应性(p:0.001)。在OTSCC中,在实质中的T-bet+与基质中的IFN-γ之间观察到正相关且具有统计学意义(r:0.388;p:0.034),除了在LLSCC中与基质(r:0.411;p:0.024)相比的实质中的T-bet和在实质和基质中的IFN-γ(r:0.775;p:0.000)之间存在统计学上显著的正相关。在OTSCC中观察到更高的T-bet+,尽管在LLSCCs中检测到更高的IFN-γ。
结论:因此,我们建议,即使LLSCC提出了较低的T-bet+,这些病变中有利的微环境导致T-bet+表达性激活IFN-γ,对Th1分化和抗肿瘤活性有很大作用,which,诚然,表现出较少的攻击性行为,再次加强这种细胞因子的重要作用及其在抗癌策略中的用途。
BACKGROUND: Evaluate the immunohistochemical expression of T-bet and IFN-γ in lower lip (LLSCC) and oral tongue squamous cell carcinoma (OTSCC), verifying the presence of Th1 responses in lesions with different clinical conditions.
METHODS: Thirty OTSCC and 30 LLSCC were analyzed by immunohistochemistry. T-bet was quantitatively assessed by parenchyma cell and stroma quantification, and IFN-γ was semi-quantitatively analyzed: 1:0-25%; 2:26-50%; 3:51-75%; 4:> 75% immunopositive cells. Histological differentiation degrees were categorized as well differentiated (WD), moderately differentiated (MD), or poorly differentiated (PD).
RESULTS: OTSCC presented the highest number of T-bet+, parenchyma (p: 0.006), stroma (p: 0.156), parenchyma/stroma (p: 0.015), with no relationship to histological malignancy grade. IFN-γ higher concentrations in LLSCC were detected in parenchyma, stroma and in parenchyma/stroma (p: 0.000), as well as greater immunoreactivity in WD and MD (p: 0.001). In OTSCC, a positive and statistically significant correlation was observed between T-bet+ in parenchyma and IFN-γ in stroma(r: 0.388; p: 0.034), in addition to a statistically significant positive correlation between T-bet in parenchyma compared to stroma(r: 0.411; p: 0.024) and for IFN-γ in both parenchyma and stroma(r: 0.775; p: 0.000) in LLSCC. Higher T-bet+ was observed in OTSCCs, although higher IFN-γ was detected in LLSCCs.
CONCLUSIONS: Thus, we suggest that, even though LLSCC presented lower T-bet+, the favorable microenvironment in these lesions led to an expressive activation of IFN-γ by T-bet+, considerably acting on Th1 differentiation and in antitumor activity, which, admittedly, present less aggressive behavior, reinforcing once again the important role of this cytokine and its use in strategy to fight cancer.