目的:小鼠模型被广泛用于增强我们对心脏病的认识。这项研究的目的是研究使用心脏磁共振(CMR)特征跟踪(CMR42,加拿大)在小鼠中测量的应变参数的可重复性。
方法:我们回顾性分析了来自13个C57BL/6B6的黑血CMR数据集。SJL-CD45.1小鼠(N=10雌性,N=3男性),先前成像。圆周,纵向,和径向(Ecc,Ell,Err,分别)在左心室的中室区域测量应变参数。评估了收缩末期(ES)和峰值应变的观察者内部和观察者之间的可重复性。
结果:与峰值应变相比,ES应变具有更大的组内相关系数(ICC)值,对于观察者内和观察者间的可重复性研究。具体来说,观察者内部研究对所有三个ES应变参数都显示出极好的可重复性,即,Ecc(ICC0.95,95%CI0.83-0.98),Ell(ICC0.90,95%CI0.59-0.97),和错误(ICC0.92,95%CI0.73-0.97)。观察者间研究也是如此,即,Ecc(ICC0.92,95%CI0.60-0.98),Ell(ICC0.76,95%CI0.33-0.93),和错误(ICC0.93,95%CI0.68-0.98)。此外,变异系数值均<10%。
结论:这项初步研究的结果对所有ES菌株参数均具有良好的可重复性,峰值应变参数具有良好到优异的重现性。此外,所有ES应变参数的ICC值均大于峰值应变。总的来说,这些结果表明,CMR42软件和黑血电影图像的特征跟踪可以可靠地用于评估小鼠的毒株模式.
Mouse models are widely utilized to enhance our understanding of cardiac disease. The goal of this study is to investigate the reproducibility of strain parameters that were measured in mice using cardiac magnetic resonance (CMR) feature-tracking (CMR42, Canada).
We retrospectively analyzed black-blood CMR datasets from thirteen C57BL/6 B6.SJL-CD45.1 mice (N = 10 female, N = 3 male) that were imaged previously. The circumferential, longitudinal, and radial (Ecc, Ell, and Err, respectively) parameters of strain were measured in the mid-ventricular region of the left ventricle. Intraobserver and interobserver reproducibility were assessed for both the end-systolic (ES) and peak strain.
The ES strain had larger intraclass correlation coefficient (ICC) values when compared to peak strain, for both the intraobserver and interobserver reproducibility studies. Specifically, the intraobserver study showed excellent reproducibility for all three ES strain parameters, namely, Ecc (ICC 0.95, 95% CI 0.83-0.98), Ell (ICC 0.90, 95% CI 0.59-0.97), and Err (ICC 0.92, 95% CI 0.73-0.97). This was also the case for the interobserver study, namely, Ecc (ICC 0.92, 95% CI 0.60-0.98), Ell (ICC 0.76, 95% CI 0.33-0.93), and Err (ICC 0.93, 95% CI 0.68-0.98). Additionally, the coefficient of variation values were all < 10%.
The results of this preliminary study showed excellent reproducibility for all ES strain parameters, with good to excellent reproducibility for the peak strain parameters. Moreover, all ES strain parameters had larger ICC values than the peak strain. In general, these results imply that feature-tracking with CMR42 software and black-blood cine images can be reliably used to assess strain patterns in mice.