背景:突触结合蛋白11(SYT11)在神经元囊泡运输和胞吐中起关键作用。然而,没有针对各种癌症的独立预后研究.在这项研究中,我们旨在总结SYT11在各种肿瘤类型中的临床意义和分子前景。
方法:使用几个可用的公共数据库,我们研究了SYT11在不同肿瘤类型中的异常表达及其与预后的潜在临床关联,甲基化分析,免疫浸润,基因富集分析,和蛋白质-蛋白质相互作用分析,并确定了共同的途径。
结果:TCGA和基因型组织表达(GTEx)显示SYT11在肿瘤和相应的正常组织中广泛表达。生存分析显示SYT11表达与7种癌症类型的预后相关。此外,SYT11mRNA表达不受启动子甲基化的影响,但受某些miRNA调控,与癌症患者预后相关。体外实验进一步验证了SYT11与miR-19a-3p在人大肠癌中的表达呈负相关,肺,和肾癌细胞系。此外,SYT11异常表达与免疫浸润显著相关。通路富集分析显示SYT11的生物学和分子过程与网格蛋白介导的内吞作用有关,RhoGTPase信号传导,和细胞运动相关的功能。
结论:我们的结果为SYT11在各种癌症类型中的作用提供了清晰的理解,并提示SYT11可能具有预后和临床意义。
BACKGROUND: Synaptotagmin 11 (SYT11) plays a pivotal role in neuronal vesicular trafficking and exocytosis. However, no independent prognostic studies have focused on various cancers. In this study, we aimed to summarize the clinical significance and molecular landscape of SYT11 in various tumor types.
METHODS: Using several available public databases, we investigated abnormal SYT11 expression in different tumor types and its potential clinical association with prognosis, methylation profiling, immune infiltration, gene enrichment analysis, and protein-protein interaction analysis, and identified common pathways.
RESULTS: TCGA and Genotype-Tissue Expression (GTEx) showed that SYT11 was widely expressed across tumor and corresponding normal tissues. Survival analysis showed that SYT11 expression correlated with the prognosis of seven cancer types. Additionally, SYT11 mRNA expression was not affected by promoter methylation, but regulated by certain miRNAs and associated with cancer patient prognosis. In vitro experiments further verified a negative correlation between the expression of SYT11 and miR-19a-3p in human colorectal, lung, and renal cancer cell lines. Moreover, aberrant SYT11 expression was significantly associated with immune infiltration. Pathway enrichment analysis revealed that the biological and molecular processes of SYT11 were related to clathrin-mediated endocytosis, Rho GTPase signaling, and cell motility-related functions.
CONCLUSIONS: Our results provide a clear understanding of the role of SYT11 in various cancer types and suggest that SYT11 may be of prognostic and clinical significance.