背景:缺血再灌注损伤(IRI)是一种影响移植存活的现象。我们研究的目的是检查IRI在暴露于长时间热缺血的同基因和同种异体肌肉和皮肤移植模型中的作用。
方法:使用48只Lewis大鼠和16只Brown-Norway大鼠创建四组:等基因腹股沟皮瓣移植(IST),等基因腓肠肌皮瓣移植(IMT),同种异体腹股沟皮瓣移植(AST),同种异体腓肠肌皮瓣移植(AMT)。在术后第1、7、21、35、63、100和120天测量所有组的丙二醛(MDA)和超氧化物歧化酶(SOD)水平。在术后第7、21、35、63、100和120天,在同种异体组中评估外周血中的供体特异性嵌合状态(DSC)。在术后第1、7和120天评估所有组的microRNA-21和microRNA-205水平。在研究结束时,进行了组织病理学检查.
结果:在MDA和SOD水平方面,各组之间存在统计学上的显着差异。在AMT组中检测到DSC。观察到microRNA-205的显着增加,尤其是AMT组。肌肉移植组之间的功能肌肉单位数量没有显着差异。
结论:在AMT组中存在DSC以及在IMT和AMT组中功能肌肉单位数量缺乏显著差异是值得注意的发现。
BACKGROUND: Ischemia-reperfusion injury (IRI) is a phenomenon that affects transplant survival. The aim of our study was to examine the effects of IRI in isogenic and allogeneic muscle and skin transplantation models exposed to prolonged warm ischemia.
METHODS: Forty-eight Lewis rats and 16 Brown-Norway rats were used to create four groups: Isogenic Inguinal Flap Transplantation (IST), Isogenic Gastrocnemius Muscle Flap Transplantation (IMT), Allogeneic Inguinal Flap Transplantation (AST), and Allogeneic Gastrocnemius Muscle Flap Transplantation (AMT). Malonyldialdehyde (MDA) and superoxide dismutase (SOD) levels were measured on postoperative days 1, 7, 21, 35, 63, 100, and 120 in all groups. Donor-specific chimerism (DSC) in peripheral blood was evaluated in the allogeneic groups on postoperative days 7, 21, 35, 63, 100, and 120. The microRNA-21 and microRNA-205 levels were evaluated on postoperative days 1, 7, and 120 in all groups. At the end of the study, a histopathological examination was performed.
RESULTS: A statistically significant difference was found between the groups in terms of MDA and SOD levels. DSC was detected in the AMT group. A significant increase in microRNA-205 was observed, especially in the AMT group. There was no significant difference in the number of functional muscle units between the muscle transplantation groups.
CONCLUSIONS: The presence of DSC in the AMT group and the lack of a significant difference in the number of functional muscle units in the IMT and AMT groups are noteworthy findings.