水分亏缺胁迫降低了大田作物的作物产量,包括向日葵,在任何成长阶段。作为回应,大多数植物激活激素和基因表达模式以减轻损害。在这项研究中,我们评估了两个向日葵(HelianthusannuusL.)自交系的生理和基因转录水平的变化-一个敏感(B59系)和一个耐水(B71)-响应水分胁迫,通过使用甘露醇来模拟缺水条件,在两个向日葵系中提供中等压力。在这种胁迫下对各种植物激素积累的分析表明,茉莉酸(JA)在两个品系的芽中均显着增加。同样,水杨酸(SA)在两个系的芽中都增加,尽管它也在B71根部积累。此外,脱落酸(ABA)和吲哚-3-乙酸(IAA)在B59芽中显示出相当大的增加。关于JA和SA途径,在两个品系的芽和B71的根中,WRKY70转录水平均较高。B59品系显示与ABA途径相关的基因(XERICO)和与IAA相关的基因(ARF9和ARF16基因)的过度转录。B71线,另一方面,同时触发了JA,SA和ABA激素途径响应这种胁迫条件。ABA和JA激素途径激活了不同的TFs,如RD20,RD22,RD26,ANAC19和ANAC29,通过MYC2。JA和SA激素途径均激活WRKY70转录因子。总之,每个细胞系都触发了激素和转录途径,以响应水分胁迫,虽然在不同的强度。结果表明,JA的激素途径,SA,IAA和ABA,以及它们的主要相关基因,在向日葵幼苗的早期生长阶段响应水分亏缺而被激活,减轻损害。
■在线版本包含补充材料,可在10.1007/s12298-024-01497-8获得。
Water deficit stress reduces crop yield in field crops, including sunflowers, at any growth stage. In response, most plants activate hormonal and gene expression patterns to mitigate damage. In this study, we evaluated changes in the physiological and gene transcription levels of two
sunflower (Helianthus annuus L.) inbred lines -one sensitive (B59 line) and one water stress-tolerant (B71)-in response to water stress, by using mannitol to simulate water deficit conditions, which provides moderate stress in both
sunflower lines. The analyses of the accumulation of various phytohormones under this stress revealed that Jasmonic acid (JA) significantly increased in the shoots of both lines. Similarly, Salicylic acid (SA) increased in the shoots of both lines, although it also accumulated in B71 roots. In addition, Abscisic acid (ABA) and Indole-3-acetic acid (IAA) showed a considerable increase in the B59 shoots. Regarding the JA and SA pathways, the WRKY70 transcription levels were higher in the shoots of both lines and the roots of B71. The B59 line showed overtranscription of a gene related to the ABA pathway (XERICO) and genes associated with IAA (ARF9 and ARF16 genes). The B71 line, on the other hand, simultaneously triggered the JA, SA and ABA hormonal pathways in response to this stress condition. The ABA and JA hormonal pathways activated different TFs, such as RD20, RD22, RD26, ANAC19 and ANAC29, through MYC2. Both the JA and SA hormonal pathways activated the WRKY70 transcription factor. Altogether, each line triggered the hormonal and transcriptional pathways in response to water stress, although at varying intensities. The results suggest that the hormonal pathways of JA, SA, IAA and ABA, along with their primary associated genes, are activated in response to water deficit at the early growth stage in
sunflower seedlings, which mitigates damage.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s12298-024-01497-8.