随着人们对食品包装的兴趣日益浓厚,抗菌活性包装材料得到了广泛的研究关注。在这次调查中,我们通过掺入壳寡糖(COS)和鱼皮明胶(FSG)纳米纤维膜开发了具有抗菌和抗氧化性能的水凝胶包装材料,容易吸收水并表现出溶胀特性。采用静电纺丝技术制备纳米纤维膜,在FSG中嵌入COS,随后通过添加葡萄糖促进的美拉德反应交联。导电性的行为,粘度,并分析了纺丝溶液中的表面张力,以了解它们的变化规律。扫描电子显微镜(SEM)结果表明,交联的COS/FSG纳米纤维膜具有均匀而无序的纤维结构,随着COS含量的增加,纳米纤维的直径增加。值得注意的是,当COS含量达到25%时,COS/FSG纳米纤维膜(CF-C-25)表现出437.16±63.20nm的合适纤维直径。此外,涉及葡萄糖补充的热交联过程增强了CF-C-25的疏水性。水合时,CF-H-25水凝胶显示出独特的多孔结构,表现出954%的显著溶胀率。值得注意的是,包含COS显着增强了基于水凝胶的纳米纤维膜的抗菌和抗氧化性能。CF-H-25对大肠杆菌表现出令人印象深刻的90.56±5.91%的生长抑制作用,再加上优异的抗氧化能力。在继续,我们对菌落总数进行了全面分析,pH值,TVB-N,和cru鱼的TBA。CF-H-25水凝胶被证明可以有效地将cru鱼的保质期延长2-4天,表明其作为水产品包装的可食用膜的潜在应用。
Antibacterial and active packaging materials have gained significant research attention in response to the growing interest in food packaging. In this investigation, we developed hydrogel packaging materials with antibacterial and antioxidant properties by incorporating chitooligosaccharide (COS) and fish skin gelatin (FSG) nanofiber membranes, which readily absorbed water and exhibited swelling characteristics. The nanofiber membranes were fabricated by electrospinning technology, embedding COS within FSG, and subsequently crosslinked through the Maillard reaction facilitated by the addition of glucose. The behavior of conductivity, viscosity, and surface tension in the spinning solutions was analyzed to understand their variation patterns. Scanning electron microscopy (SEM) results revealed that the crosslinked COS/FSG nanofiber membranes possessed a uniform yet disordered fiber structure, with the diameter of the nanofibers increasing as the COS content increased. Remarkably, when the COS content reached 25 %, the COS/FSG nanofiber membranes (CF-C-25) exhibited a suitable fiber diameter of 437.16 ± 63.20 nm. Furthermore, the thermal crosslinking process involving glucose supplementation enhanced the hydrophobicity of CF-C-25. Upon hydration, the CF-H-25 hydrogel displayed a distinctive porous structure, exhibiting a remarkable swelling rate of 954 %. Notably, the inclusion of COS significantly augmented the antibacterial and antioxidant properties of the hydrogel-based nanofiber membranes. CF-H-25 demonstrated an impressive growth inhibition of 90.56 ± 5.91 % against E. coli, coupled with excellent antioxidant capabilities. In continuation, we performed a comprehensive analysis of the total colony count, pH, TVB-N, and TBA of crucian carp. The CF-H-25 hydrogel proved highly effective in extending the shelf life of crucian carp by 2-4 days, suggesting its potential application as an edible membrane for aquatic product packaging.