探讨遮荫处理对葡萄浆果的影响,在不同的透光率(100%(CK),75%(A),50%(B),25%(C),0%(D))。观察到这种处理延缓了葡萄浆果的成熟。葡萄浆果的个体重量,以及果糖的含量,葡萄糖,可溶性糖,和浆果中的有机酸,在开花后90、100和125天测量(DAF90,DAF100,DAF125)。结果表明,遮光处理降低了葡萄果实中的糖含量;与其他处理相比,CK处理中的果糖和葡萄糖含量较高。它们随着遮光处理的持续时间而增加。相反,蔗糖含量表现出相反的趋势。此外,随着葡萄浆果重量的增加,浆果中可溶性固形物和可溶性糖的含量也增加了,而可滴定酸度下降。此外,根据转录组测序数据,在光合作用-天线蛋白途径中鉴定出16个差异表达基因(DEGs)。相关分析表明,VIT_08s0007g02190(Lhcb4)和VIT_15s0024g00040(Lhca3)基因的表达水平与DAF100时浆果中的糖含量呈正相关,而在DAF125时呈负相关。qRT-PCR结果证实了相关性分析。这表明遮荫葡萄簇抑制了葡萄浆果光合作用-天线蛋白途径中基因的表达,导致糖含量下降。这一发现有助于更深入地了解葡萄集群遮荫对浆果品质的影响机制。为提高葡萄果实品质提供重要科学依据。
To explore the impact of shade treatment on grape berries, \'Marselan\' grape berries were bagged under different light transmission rates (100% (CK), 75% (A), 50% (B), 25% (C), 0% (D)). It was observed that this treatment delayed the ripening of the grape berries. The individual weight of the grape berries, as well as the content of fructose, glucose, soluble sugars, and organic acids in the berries, was measured at 90, 100, and 125 days after flowering (DAF90, DAF100, DAF125). The results revealed that shading treatment reduced the sugar content in grape berries; the levels of fructose and glucose were higher in the CK treatment compared to the other treatments, and they increased with the duration of the shading treatment. Conversely, the sucrose content exhibited the opposite trend. Additionally, as the weight of the grape berries increased, the content of soluble solids and soluble sugars in the berries also increased, while the titratable acidity decreased. Furthermore, 16 differentially expressed genes (DEGs) were identified in the photosynthesis-antenna protein pathway from the transcriptome sequencing data. Correlation analysis revealed that the expression levels of genes VIT_08s0007g02190 (Lhcb4) and VIT_15s0024g00040 (Lhca3) were positively correlated with sugar content in the berries at DAF100, but negatively correlated at DAF125. qRT-PCR results confirmed the correlation analysis. This indicates that shading grape clusters inhibits the expression of genes in the photosynthesis-antenna protein pathway in the grape berries, leading to a decrease in sugar content. This finding contributes to a deeper understanding of the impact mechanisms of grape cluster shading on berry quality, providing important scientific grounds for improving grape berry quality.