Camellia drupifera, a significant woody oil crop in southern China, produces oil from its fruit seeds. Understanding sugar metabolism enzyme regulation is crucial for sugar accumulation and oil synthesis in fruit organs. This study examines the dynamic changes in sugar metabolism across four developmental stages of C. drupifera fruits, from rapid fruit enlargement to oil conversion. We analyzed sugar content, enzyme activity, and transcriptomic data to identify key periods and mechanisms involved in sugar metabolism. Our findings indicate that photosynthetic products are rapidly transported from leaves to fruit organs after synthesis, with transport efficiency decreasing significantly after 48 hours. September was identified as a critical period for oil conversion, during which the highest sucrose levels and SuSy-II enzyme activity were detected in the kernels. A positive correlation was found between high expression of ten genes related to sugar metabolism enzymes and sugar transport proteins and sucrose content. Notably, the expression levels of c158337.graph_c0 (SPS), c166323.graph_c0 (SuSy), c159295.graph_c0 (SUC2-like), and c156402.graph_c0 (SUC2-like) significantly increased during the oil conversion phase.These findings provide a crucial theoretical foundation for elucidating the molecular mechanisms of sugar metabolism in C. drupifera fruits, offering insights that could enhance its economic yield.

This research assessed the quantitative and qualitative reactions of commercially grown sugar beets to four different harvest dates and their yield stability. The study followed a split-plot design within a randomized complete block design over 3 years. The main plot involved 10 sugar beet cultivars, while the subplot involved four harvest dates: August 13 (HD1), September 7 (HD2), October 3 (HD3), and November 12 (HD4). The study found that environmental conditions, genotypes, and harvest dates significantly affected various traits of sugar beet. Yearly environmental variations and their interactions with genotypes and harvest dates had substantial impacts on all measured traits at the 1% probability level. Additive main effect and multiplicative interaction analysis based on white sugar yield indicated that genotype and environment\'s additive effects, as well as the genotype-environment interaction, were significant at 1% probability level. Shokoufa and Arya, which exhibit high white sugar yield (WSY) and low first interaction principal component (IPC1) values, are identified as desirable due to their stability across different environments. Among the harvest dates in different years, the fourth and third dates showed a higher yield than the total average. Perfekta and Ekbatan exhibited high specific adaptability. According to the multi-trait stability index, Arta, Arya and Sina were recognized as stable and superior across all measured traits.

This study reports on the physicochemical and sensory attributes, total phenolic content, and antioxidant activity of 36 honey samples produced by two different stingless bee species (Tetragonula carbonaria and Tetragonula hockingsi) from Australia. The findings reveal moisture content across all samples ranges from 24.9% to 30.8% (w/w), electrical conductivity from 1.02 to 2.15 mS/cm, pH levels between 3.57 and 6.54, soluble solids from 69.2 to 75.1 °Brix, trehalulose concentrations from 6.20 to 38.2 g/100 g, fructose levels from 7.79 to 33.4 g/100 g, and glucose content from 3.36 to 26.8 g/100 g. Sucrose was undetectable in all investigated samples. In a sensory analysis involving 30 participants, Australian stingless bee honey was perceived as having a more pronounced sourness compared with New Zealand Manuka honey. The study reveals considerable variability in the composition of Australian stingless bee honey, influenced by factors such as floral availability, geographical origin, and time of harvest. It also demonstrates the presence of phenolic compounds and antioxidant activity in stingless bee honey, underlining their potential as a natural source of antioxidants. All investigated samples contain trehalulose, which supports the findings of other recent studies that propose this unusual disaccharide as a marker compound of stingless bee honey.

Soluble sugar and organic acids are key determinants of fruit organoleptic quality and directly affect the commodity value and economic returns of fruit crops. We performed whole-genome sequencing of the apple varieties Gala and Xiahongrou, along with their F1 hybrids, to construct a high-density bin map. Our quantitative genetic analysis pinpointed 53 quantitative trait loci (QTLs) related to 11 sugar and acid traits. We identified a candidate gene, MdNADP-ME, responsible for malate degradation, in a stable QTL on linkage group 15. Sequence analysis revealed an A/C SNP in the promoter region (MEp-799) that influences binding of the MdMYB2 transcription factor, thereby affecting MdNADP-ME expression. In our study of various apple genotypes, this SNP has been demonstrated to be linked to malate and fructose levels. We also developed a dCAPS marker associated with fruit fructose content. These results substantiate the role of MdNADP-ME in maintaining the equilibrium between sugar and acid contents in apple fruits.

To explore the impact of shade treatment on grape berries, \'Marselan\' grape berries were bagged under different light transmission rates (100% (CK), 75% (A), 50% (B), 25% (C), 0% (D)). It was observed that this treatment delayed the ripening of the grape berries. The individual weight of the grape berries, as well as the content of fructose, glucose, soluble sugars, and organic acids in the berries, was measured at 90, 100, and 125 days after flowering (DAF90, DAF100, DAF125). The results revealed that shading treatment reduced the sugar content in grape berries; the levels of fructose and glucose were higher in the CK treatment compared to the other treatments, and they increased with the duration of the shading treatment. Conversely, the sucrose content exhibited the opposite trend. Additionally, as the weight of the grape berries increased, the content of soluble solids and soluble sugars in the berries also increased, while the titratable acidity decreased. Furthermore, 16 differentially expressed genes (DEGs) were identified in the photosynthesis-antenna protein pathway from the transcriptome sequencing data. Correlation analysis revealed that the expression levels of genes VIT_08s0007g02190 (Lhcb4) and VIT_15s0024g00040 (Lhca3) were positively correlated with sugar content in the berries at DAF100, but negatively correlated at DAF125. qRT-PCR results confirmed the correlation analysis. This indicates that shading grape clusters inhibits the expression of genes in the photosynthesis-antenna protein pathway in the grape berries, leading to a decrease in sugar content. This finding contributes to a deeper understanding of the impact mechanisms of grape cluster shading on berry quality, providing important scientific grounds for improving grape berry quality.

Sugar content is an essential indicator for evaluating crisp pear quality and categorization, being used for fruit quality identification and market sales prediction. In this study, we paired a support vector machine (SVM) algorithm with genetic algorithm optimization to reliably estimate the sugar content in crisp pears. We evaluated the spectral data and actual sugar content in crisp pears, then applied three preprocessing methods to the spectral data: standard normal variable transformation (SNV), multivariate scattering correction (MSC), and convolution smoothing (SG). Support vector regression (SVR) models were built using processing approaches. According to the findings, the SVM model preprocessed with convolution smoothing (SG) was the most accurate, with a correlation coefficient 0.0742 higher than that of the raw spectral data. Based on this finding, we used competitive adaptive reweighting (CARS) and the continuous projection algorithm (SPA) to select key representative wavelengths from the spectral data. Finally, we used the retrieved characteristic wavelength data to create a support vector machine model (GASVR) that was genetically tuned. The correlation coefficient of the SG-GASVR model in the prediction set was higher by 0.0321 and the root mean square prediction error (RMSEP) was lower by 0.0267 compared with those of the SG-SVR model. The SG-CARS-GASVR model had the highest correlation coefficient, at 0.8992. In conclusion, the developed SG-CARS-GASVR model provides a reliable method for detecting the sugar content in crisp pear using hyperspectral technology, thereby increasing the accuracy and efficiency of the quality assessment of crisp pear.

The objective of the present study was to evaluate whether the content of sugar, protein, fat, or fibre in commercially available and specially formulated plant-based beverages (oat, soya and pea) influences the growth rates of Listeria. Beverages were inoculated with a strain cocktail of Listeria (approximately 1 × 103 CFU/mL), and the data demonstrated that Listeria could proliferate in all tested beverages. Moreover, varying concentrations of naturally occurring or added sugar (0-3.3%), protein (3.3-5%), fat (1.1-3.5%) and added fibre (0-1.5%) did not have a statistically significant (p > 0.05) impact on the growth rates of Listeria in the tested plant-based beverages. These data suggest that the wide variety of commercial plant-based beverages serve as an ideal medium for the growth of Listeria irrespective of product composition. All the various products tested provided sufficient nutrients to support at least a 2.6-log increase of Listeria within 16 h at room temperature, with some beverages supporting a 3-log increase. Therefore, these data highlight the importance of careful storage and handling of these increasingly varied and popular products.

The purpose of this study was to determine the content of certain phenolic compounds, antioxidant activity, pressing efficiency, extract content, and sugars in celeriac juices obtained from the pulp after α-amylase treatment from Aspergillus oryzae. The test material consisted of peeled and unpeeled celery pulp kept at a temperature of 25 °C with and without the enzyme for a period of 30 and 60 min. The juices obtained from them were analyzed for the content of selected phenolic acids and flavonoids using the UPLC-PDA-ESI-MS/MS method, for antioxidant activity measured using the ABTS˙+ and DPPH˙ method, and for the total polyphenol content using the F-C method. Additionally, the juice pressing efficiency, the extract content using the refractometer method, and the sugar content using the HPLC method were checked. Significantly higher antioxidant activity, pressing yield, and average content of caffeic acid glucoside, quinic acid, kaempferol-3,7-di-O-glucoside, and chrysoeriol-7-O-apiosylglucoside were obtained in juices from peeled celery. Maceration of the pulp with amylase resulted in a significant reduction in antioxidant activity compared to control samples. An is-total increase of 17-41% in total flavonoid content was observed in all juices tested after treatment with the enzyme for 30 and 60 min, and the phenolic acid content increased by 4-41% after treatment of the pulp with amylase for 60 min. The 60 min holding of the pulp at 25 °C, including with the enzyme, was shown to decrease the antioxidant activity and the content of quinic acid, ferulic acid, and chrysoriol-7-O-apiose-glucoside in the juices tested compared to the samples held for 30 min, while the content of other phenolic acids and flavonoids increased. In addition, after 60 min of enzymatic maceration, the pressing yield of the juices increased.

Kiwiberry (Actinidia arguta) is a perennial fruit tree belonging to the family Actinidiaceae. Kiwiberries are known to have an extremely high concentration of sugars, phenolics, flavonoids, and vitamin C, and possess delicious taste and health-promoting properties. Numerous studies have focused on kiwiberry fruits, demonstrating that they possess a higher phytochemical content and greater antioxidant activities than other berry fruits. The purpose of this study was to compare the phytochemical content and antioxidant potential of leaf, stem, root, and fruit extracts from twelve kiwiberry cultivars grown in Wonju, Korea, characterized by a Dwa climate (Köppen climate classification). In most kiwiberry cultivars, the total phenolic (TPC) and total flavonoid (TFC) phytochemical content was significantly higher in leaf and stem tissues, while the roots exhibited higher antioxidant activity. In fruit tissues, the TPC and TFC were higher in unripe and ripe kiwiberry fruits, respectively, and antioxidant activity was generally higher in unripe than ripe fruit across most of the cultivars. Based on our results, among the 12 kiwiberry cultivars, cv. Daebo and cv. Saehan have a significantly higher phytochemical content and antioxidant activity in all of the tissue types, thus having potential as a functional food and natural antioxidant.

The growing beekeeping industry in South Korea has led to the establishment of new honey plant complexes. However, studies on honey production from each species are limited. This study aimed to assess the honey production potential of various Brassica napus cultivars and two wildflower species. The nectar characteristics of B. napus varied significantly among the cultivars. Absolute sugar concentrations differed among the cultivars, but sugar composition ratios were similar. In contrast, the amino acid content remained relatively uniform regarding percentage values, irrespective of the absolute concentrations. Estimations of honey potential production per hectare (kg/ha) resulted in the following ranking among cultivars: \'JM7003\' (107.1) > \'YS\' (73.0) > \'JM7001\' (63.7) > \'TL\' (52.7) > \'TM\' (42.4). The nectar volume of Pseudolysimachion rotundum var. subintegrum and Leonurus japonicus increased during the flowering stage. P. rotundum var. subintegrum was sucrose-rich and L. japonicus was sucrose-dominant. Both species predominantly contained phenylalanine, P. rotundum var. subintegrum had glutamine as the second most abundant amino acid, and L. japonicus had tyrosine. The honey production potential was 152.4 kg/ha for P. rotundum var. subintegrum and 151.3 kg/ha for L. japonicus. These findings provide a basis for identifying food resources for pollinators and selecting plant species to establish honey plant complexes.