这项研究调查了三氯半乳蔗糖如何影响兔肠和盲肠微生物活性,血液参数,增长业绩,car体特征,和消化率。总的来说,来自APRI品系的160只重563.29gm的5周龄兔子被随机分配到四个实验组,每组重复4只,雄性5只,雌性5只。使用四个实验组,如下:SUC1,SUC2和SUC3每天在水中获得75、150和300毫克三氯蔗糖/千克体重,而对照组则吃不补充的基础饮食。结果显示,对照组和SUC1组均显著(p<0.05)增加每日体重增加和最终体重。三氯蔗糖的添加显著提高了饲料转化率(p<0.05),降低了日采食量(gm/d)。实验组在死亡率方面没有显著差异。此外,三氯蔗糖处理对养分消化率没有显着影响,除了粗蛋白质消化,显著降低(p<0.05)。此外,不改变肝脏或肾脏功能,三氯半乳蔗糖给药显著(p<0.05)降低血清葡萄糖和甘油三酯水平,同时增加总脂质,胆固醇,与对照组相比,丙二醛。此外,蔗糖的添加导致细菌总数显着增加(p<0.05),乳酸菌,和梭菌属。,大肠杆菌数量减少。使用16SrRNA数据的进一步分析显示,三氯蔗糖上调乳杆菌基因的表达,但不上调梭菌或大肠杆菌的表达(p<0.05)。因此,可以得出结论,补充家兔三氯半乳蔗糖可以改变肠道微生物群,提高有益菌和饲料转化率,无副作用。此外,三氯半乳蔗糖可降低血糖并加剧高胆固醇血症,应谨慎使用。
This study investigated how
sucralose influenced rabbit intestine and caecal microbial activity, blood parameters, growth performance, carcass characteristics, and digestibility. In total, 160 5-week-old rabbits from the APRI line weighing 563.29 gm were randomly assigned to four experimental groups with four replicates-5 males and 5 females in each. Four experimental groups were used, as follows: SUC1, SUC2, and SUC3 got 75, 150, and 300 mg of
sucralose/kg body weight in water daily, while the control group ate a basal diet without supplements. The results showed that both the control and SUC1 groups significantly (p < 0.05) increased daily weight gain and final body weight.
Sucralose addition significantly improved feed conversion ratio (p < 0.05) and decreased daily feed intake (gm/d). The experimental groups do not significantly differ in terms of mortality. Furthermore, nutrient digestibility was not significantly affected by sucralose treatment, with the exception of crud protein digestion, which was significantly reduced (p < 0.05). Additionally, without altering liver or kidney function,
sucralose administration dramatically (p < 0.05) decreased blood serum glucose and triglyceride levels while increasing total lipids, cholesterol, and malonaldehyde in comparison to the control group. Furthermore, the addition of sucrose resulted in a significant (p < 0.05) increase in the count of total bacteria, lactobacillus, and Clostridium spp., and a decrease in the count of Escherichia coli. Further analysis using 16S rRNA data revealed that sucralose upregulated the expression of lactobacillus genes but not that of Clostridium or E. Coli bacteria (p < 0.05). Therefore, it could be concluded that sucralose supplementation for rabbits modifies gut microbiota and boosts beneficial bacteria and feed conversion ratios without side effects. Moreover,
sucralose could decrease blood glucose and intensify hypercholesterolemia and should be used with caution for human consumption.