■火山起源的活跃热液喷口在极端条件下提供了地球上生命的显着表现,这可能会影响我们对其他陆地身体可居住性的理解。
■这里,我们首次对从圣托里尼-科伦博火山田收集的海底样品进行了Illumina细菌和古细菌群落测序。收集并处理了总共19个(3米长)重力取料器,用于微生物群落分析。
■从所有样品总共产生的6,46,671个V4序列中,总共确定了10,496个不同的操作分类单位(OTU),它们被分配给40个细菌和9个古细菌门以及14个候选部门。平均而言,所有样品中最丰富的门是氯氟菌(Chloroflexota)(24.62%),其次是变形杆菌(假单胞菌)(11.29%),厚壁菌(芽孢杆菌)(10.73%),Crenarchaeroota(Thermoproteota)(8.55%),和酸杆菌(酸杆菌)(8.07%)。在属一级,共有286个已知属和候选属主要由芽孢杆菌属组成,热曲肌,Desulfatiglans,假交替单胞菌,和假单胞菌.
■在大多数车站,较深层的Chao1值与地表沉积物样品相当,表明这些生态系统的地下多样性很高。基于100个最丰富的OTU的热图分析,根据采样站的地理位置对采样站进行分组,将两个最热的站放在一起(最高99°C)。这一结果表明,活跃的科伦博火山口内的这个特定区域创造了一个独特的生态位,在那里,具有适应策略来承受热应力的微生物可以茁壮成长,例如形成内生孢子的Firmicutes。
UNASSIGNED: Active hydrothermal vents of volcanic origin provide a remarkable manifestation of life on Earth under extreme conditions, which may have consequences for our understanding of habitability on other terrestrial bodies as well.
UNASSIGNED: Here, we performed for the first time Illumina sequencing of bacterial and archaeal communities on sub-seafloor samples collected from the Santorini-Kolumbo volcanic field. A total of 19 (3-m long) gravity corers were collected and processed for microbial community analysis.
UNASSIGNED: From a total of 6,46,671 produced V4 sequences for all samples, a total of 10,496 different Operational Taxonomic Units (OTUs) were identified that were assigned to 40 bacterial and 9 archaeal phyla and 14 candidate divisions. On average, the most abundant phyla in all samples were Chloroflexi (Chloroflexota) (24.62%), followed by Proteobacteria (Pseudomonadota) (11.29%), Firmicutes (Bacillota) (10.73%), Crenarchaeota (Thermoproteota) (8.55%), and Acidobacteria (Acidobacteriota) (8.07%). At the genus level, a total of 286 known genera and candidate genera were mostly dominated by members of Bacillus, Thermoflexus, Desulfatiglans, Pseudoalteromonas, and Pseudomonas.
UNASSIGNED: In most of the stations, the Chao1 values at the deeper layers were comparable to the surface sediment samples denoting the high diversity in the subsurface of these ecosystems. Heatmap analysis based on the 100 most abundant OTUs, grouped the sampling stations according to their geographical location, placing together the two hottest stations (up to 99°C). This result indicates that this specific area within the active Kolumbo crater create a distinct niche, where microorganisms with adaptation strategies to withstand heat stresses can thrive, such as the endospore-forming Firmicutes.