■慢性硬化性唾液腺炎和IgG4相关疾病(IgG4-RD)的关联导致下颌下腺炎症中IgG4阳性的更频繁的鉴定,还揭示了非特异性炎症中IgG4的过度表达。这些发现使我们假设IgG4阳性唾液腺炎代表与IgG4-RD在组织学上重叠的连续炎症过程。病因可能不同。然而,IgG4阳性唾液腺炎和IgG4-RD中IgG4过表达的抗原仍未知.
■这里,我们调查了Toll样受体(TLR)-介导的细菌炎症在患者的下颌下腺IgG4阳性和IgG4阴性慢性炎性病变的颌下腺组织(n=61),与未发炎的颌下腺作为对照(n=4)。利用免疫组织化学,我们评估了TLR2和TLR4,脂多糖(LPS)和牙龈卟啉单胞菌特异性抗原牙龈蛋白酶R1的表达。
■我们在64个(98%)样品中观察到TLR2-和TLR4-免疫阳性。然而,TLR2和TLR4染色强度在IgG4阳性组中明显更强。在56(86%)和58(89%)样品中观察到LPS和牙龈蛋白酶R1免疫阳性,分别。LPS阳性仅位于肥大细胞样细胞中,而人参蛋白酶R1阳性仍然很少。
■在IgG4阳性的唾液腺炎中,TLR2或TLR4表达越强,可能表明这种形式的慢性唾液腺炎的组织相关因素。在整个系列中,LPS和牙龈卟啉单胞菌的免疫阳性仍然很弱。因此,革兰氏阴性菌可能不代表这些形式的慢性唾液腺炎的病原体。
UNASSIGNED: The association of chronic sclerosing sialadenitis and IgG4-related disease (IgG4-RD) has resulted in the more frequent identification of IgG4-positivity in submandibular gland inflammations, also uncovering IgG4 overexpression in nonspecific inflammations. These findings lead us to hypothesise that IgG4-positive sialadenitis represents a continuous inflammatory process overlapping histologically with IgG4-RD, possibly differing in aetiology. However, the antigen underlying IgG4 overexpression in IgG4-positive sialadenitis and IgG4-RD remains unknown.
UNASSIGNED: Here, we investigated toll-like receptor (TLR) - mediated bacterial inflammation in submandibular gland tissues of patients with IgG4-positive and IgG4-negative chronic inflammatory lesions of the submandibular gland (n = 61), with noninflamed submandibular glands serving as controls (n = 4). Utilising immunohistochemistry, we assessed the expression of TLR2 and TLR4, lipopolysaccharide (LPS) and the P. gingivalis-specific antigen gingipain R1.
UNASSIGNED: We observed TLR2- and TLR4-immunopositivity in 64 (98%) samples. However, TLR2 and TLR4 staining intensity was significantly stronger in the IgG4-positive group. LPS- and gingipain R1 immunopositivity were observed in 56 (86%) and 58 (89%) samples, respectively. LPS-positivity localised exclusively in mast cell-like cells, while gingipain R1-positivity remained scarce.
UNASSIGNED: A stronger TLR2 or TLR4 expression in IgG4-positive sialadenitis may indicate a tissue-related factor underlying this form of chronic sialadenitis. LPS- and P. gingivalis immunopositivity remained weak throughout this series. Thus, gram-negative bacteria may not represent pathogens underlying these forms of chronic sialadenitis.