背景:可变剪接(AS)是基因表达调控和蛋白质多样化的重要通道,除了真核生物中基因和蛋白质数量差异很大的一个主要原因。在植物中,U2小核核糖核蛋白B″(U2B″),剪接复合物U2snRNP的组成部分,在AS中起着重要的作用。目前,很少有研究调查植物U2B″,其机制尚不清楚。
结果:系统发育分析,包括基因和蛋白质结构,表明U2B”在植物中高度保守,通常包含两个RNA识别基序。亚细胞定位显示OsU2B″位于细胞核和细胞质中,表明它在整个细胞中具有广泛的功能。对启动子区域的元素分析表明,它对许多外部刺激有反应,包括荷尔蒙,压力,和光。随后的qPCR实验检查对压力的反应(冷,盐,干旱,和重金属镉)证实了这一发现。蛋白质相互作用的预测结果表明,其功能主要通过单一途径,主要通过与snRNP蛋白相互作用。
结论:U2B″在植物界是高度保守的,在细胞核和细胞质中发挥作用,并参与植物生长和发育的各种过程。
BACKGROUND: Alternative splicing (AS) is an important channel for gene expression regulation and protein diversification, in addition to a major reason for the considerable differences in the number of genes and proteins in eukaryotes. In plants, U2 small nuclear ribonucleoprotein B″ (U2B″), a component of splicing complex U2 snRNP, plays an important role in AS. Currently, few studies have investigated plant U2B″, and its mechanism remains unclear.
RESULTS: Phylogenetic analysis, including gene and protein structures, revealed that U2B″ is highly conserved in plants and typically contains two RNA recognition motifs. Subcellular localisation showed that OsU2B″ is located in the nucleus and cytoplasm, indicating that it has broad functions throughout the cell. Elemental analysis of the promoter region showed that it responded to numerous external stimuli, including hormones, stress, and light. Subsequent qPCR experiments examining response to stress (cold, salt, drought, and heavy metal cadmium) corroborated the findings. The prediction results of protein-protein interactions showed that its function is largely through a single pathway, mainly through interaction with snRNP proteins.
CONCLUSIONS: U2B″ is highly conserved in the plant kingdom, functions in the nucleus and cytoplasm, and participates in a wide range of processes in plant growth and development.