■目前,水稻-蘑菇轮作系统中的秸秆生物降解和土壤改良备受关注。然而,仍然缺乏关于水稻-蘑菇轮作对产量影响的研究,土壤性质和微生物演替。
■在这项研究中,不治疗(CK),以绿肥还田(GM)和稻草还田(RS)为对照,全面评估了rugogoisoynulata栽培基质还田(SRS)对土壤性质和微生物的影响。
■结果表明,水稻产量,土壤养分(有机质,有机碳,总氮,有效氮和有效钾)和土壤酶(脲酶,糖化酶,木质素过氧化物酶和漆酶)活性对水稻-蘑菇旋转具有积极响应。在年际一级,不同处理的微生物多样性差异显著,随着水稻-蘑菇轮作显著提高土壤细菌的相对α多样性指数,富集根瘤菌等有益微生物群落,芽孢杆菌和木霉为水稻生长。在水稻-蘑菇轮作过程中,土壤养分和酶活性与微生物群落显着相关。真菌-细菌共现网络是模块化的,和Latescibacota,氯氟菌,Gemmaatimonadota和Patesciacterium与土壤中养分的积累密切相关。结构方程模型(SEM)表明,真菌多样性比细菌多样性对土壤养分变化的响应更大。
■总的来说,水稻-蘑菇轮作模式提高了土壤养分和水稻产量,丰富有益微生物,保持微生物多样性。这项研究为在农业生态系统的可持续发展中使用rugosoannulata栽培基质提供了新的见解。
UNASSIGNED: Currently, straw biodegradation and soil improvement in rice-mushroom rotation systems have attracted much attention. However, there is still a lack of studies on the effects of rice-mushroom rotation on yield, soil properties and microbial succession.
UNASSIGNED: In this study, no treatment (CK), green manure return (GM) and rice straw return (RS) were used as controls to fully evaluate the effect of Stropharia rugosoannulata cultivation substrate return (SRS) on soil properties and microorganisms.
UNASSIGNED: The results indicated that rice yield, soil nutrient (organic matter, organic carbon, total nitrogen, available nitrogen and available potassium) and soil enzyme (urease, saccharase, lignin peroxidase and laccase) activities had positive responses to the rice-mushroom rotation. At the interannual level, microbial diversity varied significantly among treatments, with the rice-mushroom rotation significantly increasing the relative alpha diversity index of soil bacteria and enriching beneficial microbial communities such as Rhizobium, Bacillus and Trichoderma for rice growth. Soil nutrients and enzymatic activities were significantly correlated with microbial communities during rice-mushroom rotation. The fungal-bacterial co-occurrence networks were modular, and Latescibacterota, Chloroflexi, Gemmatimonadota and Patescibacteria were closely related to the accumulation of nutrients in the soil. The structural equation model (SEM) showed that fungal diversity responded more to changes in soil nutrients than did bacterial diversity.
UNASSIGNED: Overall, the rice-mushroom rotation model improved soil nutrients and rice yields, enriched beneficial microorganisms and maintained microbial diversity. This study provides new insights into the use of S. rugosoannulata cultivation substrates in the sustainable development of agroecosystems.