铜是一种微量元素,其电子构型为其提供了基本的结构和催化功能。然而,过量,它的高蛋白质亲和力和氧化还原催化特性都可能导致危险的后果。除了促进氧化应激,铜通过调节GABA能和谷氨酸能受体以及与多巴胺再摄取转运蛋白的相互作用而对神经传递产生了兴趣。本研究的目的是研究铜过度暴露对多巴胺水平的影响,去甲肾上腺素,和血清素,或它们在大鼠纹状体细胞外液中的主要代谢产物。使用我们以前开发的模型腹膜内注射铜,这确保了纹状体浓度过高(2mgCuCl2/kg,持续30天)。随后,在第0,15和30天通过微透析收集细胞外液.多巴胺(DA),3,4-二羟基苯乙酸(DOPAC),高香草酸(HVA),5-羟基吲哚乙酸(5-HIAA),然后通过HPLC结合电化学检测确定去甲肾上腺素(NA)水平。我们观察到治疗15天后DA和HVA的基础水平显着增加(310%和351%),30天后保持不变(358%和402%),5-HIAA的浓度没有显著变化,DOPAC,NA。铜过载导致突触DA浓度显著增加,这可能有助于在Wilson病和其他铜失调状态中观察到的心理神经学改变和氧化毒性增加。
Copper is a trace element whose electronic configuration provides it with essential structural and catalytic functions. However, in excess, both its high protein affinity and redox-catalyzing properties can lead to hazardous consequences. In addition to promoting oxidative stress, copper is gaining interest for its effects on neurotransmission through modulation of GABAergic and glutamatergic receptors and interaction with the dopamine reuptake transporter. The aim of the present study was to investigate the effects of copper overexposure on the levels of dopamine, noradrenaline, and serotonin, or their main metabolites in rat\'s
striatum extracellular fluid. Copper was injected intraperitoneally using our previously developed model, which ensured striatal overconcentration (2 mg CuCl2/kg for 30 days). Subsequently, extracellular fluid was collected by microdialysis on days 0, 15, and 30. Dopamine (DA), 3,4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), 5-hydroxyindoleacetic acid (5-HIAA), and noradrenaline (NA) levels were then determined by HPLC coupled with electrochemical detection. We observed a significant increase in the basal levels of DA and HVA after 15 days of treatment (310% and 351%), which was maintained after 30 days (358% and 402%), with no significant changes in the concentrations of 5-HIAA, DOPAC, and NA. Copper overload led to a marked increase in synaptic DA concentration, which could contribute to the psychoneurological alterations and the increased oxidative toxicity observed in Wilson\'s disease and other copper dysregulation states.