背景:在全球范围内,盐度是影响种子快速萌发的严重环境压力之一,统一的幼苗建立和植物发展限制了可持续的农业生产力。近年来,可持续农业和清洁生产战略的概念强调使用生物相容性和天然来源引入更绿色的农用化学品,以最大限度地提高作物产量和最小的生态毒理学影响。在过去的十年里,纳米技术作为跨学科科学的前沿出现,引入了纳米材料作为速效植物生长促进剂。
结果:这里,我们报告了使用壳聚糖和绿茶(CS-GTENC)作为生态耦合纳米结剂的纳米复合材料的制备,以通过引发印记来引起盐胁迫耐受性。CS-GTENC-primed(0.02、0.04和0.06%),在正常和盐胁迫(150mMNaCl)条件下,水引发和非引发(对照)小麦种子发芽。从上述种子发育的幼苗用于生理,生化和发芽研究。引发处理增加了蛋白质含量(10-12%),光合色素(Chla(4-6%),Chlb(34-36%),总Chl(7-14%)和上调抗氧化剂的机械(CAT(26-42%),胁迫条件下小麦幼苗中的POD(22-43%)。它还降低了MDA含量(65-75%)并调节了ROS的产生,从而改善了膜的稳定性。启动介导的生化属性改变导致最终发芽改善(20-22%),两种条件下的活力(4-11%)和发芽指数(6-13%)。它显著减少了平均发芽时间,建立纳米复合材料的应力绝缘作用。发芽参数的改善验证了复合处理种子中引发记忆的刺激。
结论:用纳米复合材料对种子进行预处理,使它们能够通过启动记忆来抵抗幼苗发育阶段的盐度,从而保证可持续的植物生长和高作物生产率。
BACKGROUND: Around the globe, salinity is one of the serious environmental stresses which negatively affect rapid seed germination, uniform seedling establishment and plant developments restricting sustainable agricultural productivity. In recent years, the concepts of sustainable agriculture and cleaner production strategy have emphasized the introduction of greener agrochemicals using biocompatible and natural sources to maximize crop yield with minimum ecotoxicological effects. Over the last decade, the emergence of nanotechnology as a forefront of interdisciplinary science has introduced nanomaterials as fast-acting plant growth-promoting agents.
RESULTS: Herein, we report the preparation of nanocomposite using chitosan and green tea (CS-GTE NC) as an ecofriendly nanopriming agent to elicit salt stress tolerance through priming imprints. The CS-GTE NC-primed (0.02, 0.04 and 0.06%), hydroprimed and non-primed (control) wheat seeds were germinated under normal and salt stress (150 mM NaCl) conditions. The seedlings developed from aforesaid seeds were used for physiological, biochemical and germination studies. The priming treatments increased protein contents (10-12%), photosynthetic pigments (Chl a (4-6%), Chl b (34-36%), Total Chl (7-14%) and upregulated the machinery of antioxidants (CAT (26-42%), POD (22-43%)) in wheat seedlings under stress conditions. It also reduced MDA contents (65-75%) and regulated ROS production resulting in improved membrane stability. The priming-mediated alterations in biochemical attributes resulted in improved final germination (20-22%), vigor (4-11%) and germination index (6-13%) under both conditions. It reduced mean germination time significantly, establishing the stress-insulating role of the nanocomposite. The improvement of germination parameters validated the stimulation of priming memory in composite-treated seeds.
CONCLUSIONS: Pre-treatment of seeds with nanocomposite enables them to counter salinity at the seedling development stage by means of priming memory warranting sustainable plant growth and high crop productivity.