目的:这项生物力学临床前研究旨在评估动脉同种异体移植物长期冷藏(+2/+8°C)对机械性能的影响。
方法:从多器官供体中收集股pop动脉段,并在添加抗生素的盐溶液中在2/8°C下储存12个月。使用两种不同的测试进行机械表征,其目的是定义在破裂之前样品所承受的生理模量和最大应力和应变。对于每个样品(分别为T0、T6和T12),在储存0、6和12个月后进行这些表征。为了比较,解冻后对冷冻保存的pop股骨节段进行了相同的测试。
结果:12个冷藏同种异体移植物(RA),每个分为三个部分,和10个冷冻保存的同种异体移植物(CA)进行了表征。冷藏同种异体移植物的储存时间之间的中值(四分位距[IQR])杨氏模量在统计学上没有显着差异:RAT0,164(150,188)kPa;RAT6,178(141,185)kPa;RAT12,177(149,185)kPa。CA组的中值(IQR)杨氏模量(153[141,185]kPa)与RA组无显著差异,不管储存时间。此外,不同组之间的中值(IQR)最大应力和应变值没有显着差异:对于最大应力:RAT0,1.58(1.08,2.09)MPa;RAT6,1.74(1.55,2.36)MPa;RAT12,2.25(1.87,2.53)MPa;CA,2.25(1.77,2.61)MPa;对于最大应变:RAT0,64%(50,90);RAT6,79%(63,84);RAT12,72%(65,86);CA,67%(50,95)。
结论:冷藏长达12个月似乎对人动脉同种异体移植物的机械特性没有影响。因此,这种保存方法,这将大大简化日常护理,似乎可行。在考虑在体内使用之前,正在研究其他指标以验证该保存过程的安全性。
OBJECTIVE: This biomechanical pre-clinical study aimed to assess the consequences on mechanical properties of long term cold storage (+2/+8 °C) of arterial allografts.
METHODS: Femoropopliteal arterial segments were collected from multiorgan donors and stored at +2/+8 °C for 12 months in saline solution with added antibiotics. Mechanical characterisation was carried out using two different tests, with the aim of defining the physiological modulus and the maximum stress and
strain borne by the sample before rupture. These characterisations were carried out after 0, 6, and 12 months of storage for each sample (T0, T6, and T12, respectively). For comparison, the same tests were performed on cryopreserved popliteal femoral segments after thawing.
RESULTS: Twelve refrigerated allografts (RAs), each divided into three segments, and 10 cryopreserved allografts (CAs) were characterised. The median (interquartile range [IQR]) Young\'s modulus was not statistically significantly different between the storage times for cold stored allografts: RAT0, 164 (150, 188) kPa; RAT6, 178 (141, 185) kPa; RAT12, 177 (149, 185) kPa. The median (IQR) Young\'s modulus of the CA group (153 [141, 185] kPa) showed no significant differences from the RA groups, irrespective of storage time. Furthermore, median (IQR) maximum stress and
strain values were not significantly different between the different groups: for maximum stress: RAT0, 1.58 (1.08, 2.09) MPa; RAT6, 1.74 (1.55, 2.36) MPa; RAT12, 2.25 (1.87, 2.53) MPa; CA, 2.25 (1.77, 2.61) MPa; and for maximum
strain: RAT0, 64% (50, 90); RAT6, 79% (63, 84); RAT12, 72% (65, 86); CA, 67% (50, 95).
CONCLUSIONS: Cold storage for up to 12 months appears to have no impact on the mechanical characteristics of human arterial allografts. Therefore, this preservation method, which would greatly simplify routine care, seems feasible. Other indicators are being studied to verify the safety of this preservation process before considering its use in vivo.