小菜蛾(Linnaeus)是十字花科植物的重要害虫,这在全世界都是有害的,造成严重的经济损失,其耐药性正在迅速增加。昆虫不育技术(SIT)是一种绿色控制方法,不会引起抗性。在这项研究中,采用转录组学和生物信息学方法探讨辐射对小菜蛾繁殖功能的影响,初步揭示了辐射下不育的反应机制。我们确定了3342(1682上调,1660下调),1963(1042上调,921下调)和1531(721上调,810下调)200Gy与CK(对照检查)中的差异表达基因(DEGs),400Gy对CK和400Gy对200Gy组,分别。对每组的DEG进行GO和KEGG分析。结果表明,200Gy激活了下游磷酸化途径,抑制了细胞色素p450免疫应答机制。400Gy促进蛋白质分解和吸收途径,自噬途径,等。下调基因集中在ATP等能量代谢物质的转化过程中,磷酸化信号通路,和胰岛素,而上调的基因集中在生物调控和代谢过程中。选择磷酸化通路中的8个基因进行qRT-PCR验证,结果表明,不同剂量组对磷酸化的调控方式不同。400Gy使用正反馈调节,而F1的磷酸化使用负反馈调节。
Plutella xylostella (Linnaeus) is an important pest of cruciferous plants, which is harmful all over the world, causing serious economic losses, and its drug resistance is increasing rapidly. The sterile insect technique (SIT) is a green control method and does not cause resistance. In this study, transcriptomics and bioinformatics were used to explore the effects of irradiation on the reproductive function of Plutella xylostella, and the response mechanism of sterility under irradiation was initially revealed. We identified 3342 (1682 up-regulated, 1660 down-regulated), 1963 (1042 up-regulated, 921 down-regulated) and 1531 (721 up-regulated, 810 down-regulated) differentially expressed genes (DEGs) in the 200 Gy vs CK (Control Check), 400 Gy vs CK and 400 Gy vs 200 Gy groups, respectively. GO and KEGG analyses were performed for DEGs in each group. The results showed that 200 Gy activated the downstream phosphorylation pathway and inhibited the cytochrome p450 immune response mechanism. 400 Gy promoted protein decomposition and absorption pathways, autophagy pathways, etc. Down-regulated genes were concentrated in the transformation process of energy metabolizing substances such as ATP, phosphorylation signaling pathway, and insulin, while up-regulated genes were concentrated in biological regulation and metabolic processes. Eight genes in the phosphorylation pathway were selected for qRT-PCR verification, and the results showed that the phosphorylation of different dose groups was regulated in different ways. 400 Gy used positive feedback regulation, while the phosphorylation of F1 used negative feedback regulation.