白藜芦醇(3,5,4'-三羟基-反式-二苯乙烯),葡萄和葡萄酒中常见的苯酚,在涉及不同神经递质系统改变的实验模型中被认为是保护性的。然而,研究报告白藜芦醇可能有副作用.这项研究评估了低剂量氯胺酮和白藜芦醇的关联是否可以诱导与生化改变相关的行为表现。此外,白藜芦醇和/或氯胺酮处理对单胺氧化酶(MAO)活性的影响,氧化应激标志物,还研究了大脑中的IL-6水平。雄性瑞士小鼠连续14天接受低剂量氯胺酮(20mg/kg),和白藜芦醇(10,30,或100毫克/千克)从第8天至第14天的实验期,腹膜内。运动,刻板的行为,Y-迷宫,新型识别对象测试(NORT),和社会互动进行了量化,以及MAO活性的离体分析,IL-6水平,和脑组织中的氧化应激标志物(TBARS和总硫醇水平)。氯胺酮本身减少了实验期第8天刻板行为的发作次数。白藜芦醇本身降低了开放领域的运动和探索活动,在NORT中探索新物体的时间,纹状体中的MAO-A活性并增加皮质中的IL-6水平。当小鼠用氯胺酮和白藜芦醇共处理时,这些作用减弱。用氯胺酮+白藜芦醇100mg/kg处理的小鼠的皮质中MAO-A活性降低。在氧化应激标志物中没有发现明显的改变。白藜芦醇似乎不会引起氯胺酮对行为改变的最终影响。然而,白藜芦醇本身的作用,主要是运动和探索活动,应该更好地调查。
Resveratrol (3,5,4\'-trihydroxy-trans-stilbene), a phenol commonly found in grapes and wine, has been associated as protective in experimental models involving alterations in different neurotransmitter systems. However, studies are reporting that resveratrol could have adverse effects. This study evaluated if the association of a low dose of ketamine and resveratrol could induce behavioral manifestations associated with biochemical alterations. Moreover, the effects of treatment with resveratrol and/or ketamine on monoamine oxidase (MAO) activity, oxidative stress markers, and IL-6 levels in the brain were also investigated. Male Swiss mice received a low dose of ketamine (20 mg/kg) for 14 consecutive days, and resveratrol (10, 30, or 100 mg/kg) from day 8 up to day 14 of the experimental period, intraperitoneally. Locomotor, stereotyped behavior, Y-maze, novel recognition object test (NORT), and social interaction were quantified as well as ex vivo analysis of MAO activity, IL-6 levels, and oxidative stress markers (TBARS and total thiol levels) in brain tissues. Ketamine per se reduced the number of bouts of stereotyped behavior on day 8 of the experimental period. Resveratrol per se reduced the locomotor and exploratory activity in the open field, the time of exploration of new objects in the NORT, MAO-A activity in the striatum and increased the IL-6 levels in the cortex. These effects were attenuated when the mice were co-treated with ketamine and resveratrol. There was a decrease in MAO-A activity in the cortex of mice treated with ketamine + resveratrol 100 mg/kg. No significant alterations were found in oxidative stress markers. Resveratrol does not appear to cause summative effects with ketamine on behavioral alterations. However, the effect of resveratrol per se, mainly on locomotor and exploratory activity, should be better investigated.